Publications des scientifiques de l'IRD

Alla-N'Nan O., Gondélé Bi E., Kouassi A.B., Kouassi A., Philippe R., Malaurie Bernard, Dollet M. (2014). Isolation of phytoplasma DNA from the coconut palms (Cocos nucifera L.) collected from Ghana. Journal of Experimental Biology and Agricultural Sciences, 2 (5), p. 495-500. ISSN 2320-8694.

Titre du document
Isolation of phytoplasma DNA from the coconut palms (Cocos nucifera L.) collected from Ghana
Année de publication
2014
Type de document
Article
Auteurs
Alla-N'Nan O., Gondélé Bi E., Kouassi A.B., Kouassi A., Philippe R., Malaurie Bernard, Dollet M.
Source
Journal of Experimental Biology and Agricultural Sciences, 2014, 2 (5), p. 495-500 ISSN 2320-8694
This study aimed to verify the presence of the causative agent of Lethal Yellowing which is phytoplasma in samples provided from infected coconut trees. Study was carried out by using various samples like zygotic embryo, young leaves and immature & mature inflorescences. These materials were collected from trees at the stage 1 and 2 of the disease development.. Stage 1 of disease development is characterized by leaf yellowing and the start of the falling nuts while at the stage 2 of disease development, the trees has not bear nuts longer. From infected material, DNA was extracted by three different processes and isolated DNA was amplified by PCR. 16S rRNA gene was amplified by two specific primers of phytoplama viz P1/P2 and Ghana 813/AKSR. Among the various tested materials presence of phytoplasma was reported from the mature inflorescences while the presence of the phytoplasma was not reported from the leaves and embryos of the coconut.
Plan de classement
Collection, conservation [076AMEPLA02]
Descripteurs
NOIX DE COCO ; CONSERVATION DES RESSOURCES GENETIQUES ; EMBRYON ZYGOTIQUE ; MALADIE DES PLANTES ; PATHOLOGIE VEGETALE ; AGENT PATHOGENE ; ADN ; COCOTIER
Description Géographique
GHANA
Localisation
Fonds IRD [F B010062998]
Identifiant IRD
fdi:010062998
Contact