@article{fdi:010062937,
  title = {{E}vidence of nickel ({N}i) efflux in {N}i-tolerant ectomycorhizal {P}isolithus albus isolated from ultramafic soil},
  author = {{M}ajorel, {C}larisse and {H}annibal, {L}aure and {D}ucousso, {M}. and {L}ebrun, {M}ichel and {J}ourand, {P}hilippe},
  editor = {},
  language = {{ENG}},
  abstract = {{N}ickel ({N}i)-tolerant ectomycorrhizal {P}isolithus albus was isolated from extreme ultramafic soils that are naturally rich in heavy metals. {T}his study aimed to identify the specific molecular mechanisms associated with the response of {P}. albus to nickel. {I}n presence of high concentration of nickel, {P}. albus {N}i-tolerant isolate showed a low basal accumulation of nickel in its fungal tissues and was able to perform a metal efflux mechanism. {T}hree genes putatively involved in metal efflux were identified from the {P}. albus transcriptome, and their overexpression was confirmed in the mycelium that was cultivated in vitro in the presence of nickel and in fungal tissues that were sampled in situ. {C}loning these genes in yeast provided significant advantages in terms of nickel tolerance (+ 31% {N}i {EC}50) and growth (+ 83% ) compared with controls. {F}urthermore, nickel efflux was also detected in the transformed yeast cells. {P}rotein sequence analysis indicated that the genes encoded a {P}-type-{ATP}ase, an {ABC} transporter and a major facilitator superfamily permease ({MFS}). {T}his study sheds light on a global mechanism of metal efflux by {P}. albus cells that supports nickel tolerance. {T}hese specific responses to nickel might contribute to the fungal adaptation in ultramafic soil.},
  keywords = {{ECTOMYCORHIZE} ; {NICKEL} ; {METAL} {LOURD} ; {TOLERANCE} ; {ROCHE} {ULTRABASIQUE} ; {BIOLOGIE} {MOLECULAIRE} ; {GENE} ; {EXPERIMENTATION} {IN} {VITRO} ; {ETUDE} {EXPERIMENTALE} ; {ETUDE} {COMPARATIVE} ; {EXPRESSION} {DES} {GENES} ; {TECHNIQUE} {PCR} ; {NOUVELLE} {CALEDONIE}},
  booktitle = {},
  journal = {{E}nvironmental {M}icrobiology {R}eports},
  volume = {6},
  numero = {5},
  pages = {510--518},
  ISSN = {1758-2229},
  year = {2014},
  DOI = {10.1111/1758-2229.12176},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062937},
}