@article{fdi:010062865,
  title = {{R}obustness against serum neutralization of a poliovirus type 1 from a lethal epidemic of poliomyelitis in the {R}epublic of {C}ongo in 2010},
  author = {{D}rexler, {J}.{F}. and {G}rard, {G}. and {L}ukashev, {A}.{N}. and {K}ozlovskaya, {L}. and {U}slu, {G}. and {R}eimerink, {J}. and {G}myl, {A}.{P}. and {T}aty-{T}aty, {R}. and {L}ekena-{D}ouki, {S}.{E}. and {N}gokhe, {D}. and {E}is-{H}ûbinger, {A}.{M}. and {D}iedrich, {S}. and {K}oopmans, {M}. and {L}eroy, {E}ric and {D}rosten, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n 2010, a large outbreak of poliomyelitis with unusual 47% lethality occurred in {P}ointe {N}oire, {R}epublic of {C}ongo. {V}accine-mediated immunity against the outbreak virus was never investigated. {A} wild poliovirus 1 ({WPV}1) isolated from a fatal case (termed {PV}1-{RC}2010) showed a previously unknown combination of amino acid exchanges in critical antigenic site 2 ({A}g{S}2, {VP}1 capsid protein positions (221){SAAL} ->(221){PADL}). {T}hese exchanges were also detected in an additional 11 {WPV}1 strains from fatal cases. {PV}1-{RC}2010 escaped neutralization by three different m{A}bs relevant for {A}g{S}2. {V}irus neutralization was tested in sera from fatal cases, who died before supplementary immunization (n = 24), {G}abonese recipients of recent oral polio vaccination (n = 12), routinely vaccinated {G}erman medical students (n = 34), and {G}erman outpatients tested for anti-poliovirus immunity (n = 17) on {V}ero, human rhabdomyosarcoma, and human epidermoid carcinoma 2 cells. {F}atal poliomyelitis cases gave laboratory evidence of previous trivalent vaccination. {N}eutralizing antibody titers against {PV}1-{RC}2010 were significantly lower than those against the vaccine strain {S}abin-1, two genetically distinct {WPV}1s isolated in 1965 and 2010 and two genetically distinct vaccine-derived {PV} strains. {O}f {G}erman vaccinees tested according to {W}orld {H}ealth {O}rganization protocols, 15-29% were unprotected according to their neutralization titers (<1: 8 serum dilution), even though all were protected against {S}abin-1. {P}hylogenetic analysis of the {WPV}1 outbreak strains suggested a recent introduction of virus progenitors from {A}sia with formation of separate {A}ngolan and {C}ongolese lineages. {O}nly the latter carried both critical {A}g{S}2 mutations. {A}ntigenetically variant {PV}s may become relevant during the final phase of poliomyelitis eradication in populations with predominantly vaccine-derived immunity. {S}ustained vaccination coverage and clinical and environmental surveillance will be necessary.},
  keywords = {{VIRUS} ; {VACCINATION} ; {PHYLOGENIE} ; {BIOLOGIE} {MOLECULAIRE} ; {ANTICORPS} ; {MUTATION} ; {ETUDE} {EXPERIMENTALE} ; {ETUDE} {COMPARATIVE} ; {POLIOMYELITE} ; {TECHNIQUE} {RT} {PCR} ; {CONGO} ; {POINTE} {NOIRE}},
  booktitle = {},
  journal = {{P}roceedings of the {N}ational {A}cademy of {S}ciences of the {U}nited {S}tates of {A}merica},
  volume = {111},
  numero = {35},
  pages = {12889--12894},
  ISSN = {0027-8424},
  year = {2014},
  DOI = {10.1073/pnas.1323502111},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062865},
}