@article{fdi:010062705, title = {{D}evelopment of twelve novel polymorphic microsatellite {DNA} markers for the {B}oeseman's rainbowfish ({M}elanotaenia boesemani) and tests for their cross-utility in 21 rainbowfish species from {W}est {P}apua ({I}ndonesia)}, author = {{N}ugraha, {M}. {F}. {I}. and {P}ouyaud, {L}aurent and {C}arman, {O}. and {K}adarusman, and {W}idyastuti, {U}. and {A}varre, {J}ean-{C}hristophe}, editor = {}, language = {{ENG}}, abstract = {{W}e developed 12 microsatellite markers for the endangered {B}oeseman's rainbowfish ({M}elanotaenia boesemani). {T}wenty-eight individuals from the type locality at {A}yamaru {L}ake were examined, and all loci were polymorphic with a number of alleles per locus varying from 3 to 18. {A}verage observed and expected heterozygosities were 0.681 and 0.678, respectively. {C}ross-species amplification was successfully obtained for 21 {M}elanotaenia species, with a number of alleles per locus ranging between 1 and 20. {A}verage observed and expected heterozygosities varied between 0.105 and 0.708 and 0.118-0.755, respectively. {O}nly 21 inbreeding coefficient ({F}is) values presented a significant homozygote excess among the 264 locus-by-locus calculated values. {T}ests for genotyping errors revealed that four of these 21 significant {F}is values could be explained by the presence of null alleles. {T}hese new microsatellite markers appear highly reliable for further conservation purposes or population genetic studies of the many rainbowfish endangered species.}, keywords = {{R}ainbowfish ; {M}icrosatellite {DNA} ; {M}elanotaenia boesemani ; {W}est {P}apua ; {E}ndangered species ; {PAPOUASIE} {NOUVELLE} {GUINEE}}, booktitle = {}, journal = {{E}uropean {J}ournal of {W}ildlife {R}esearch}, volume = {60}, numero = {6}, pages = {941--946}, ISSN = {1612-4642}, year = {2014}, DOI = {10.1007/s10344-014-0868-2}, URL = {https://www.documentation.ird.fr/hor/fdi:010062705}, }