%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Silipo, A.
%A Vitiello, G.
%A Gully, Djamel
%A Sturiale, L.
%A Chaintreuil, Clémence
%A Fardoux, Joël
%A Gargani, D.
%A Lee, H. I.
%A Kulkarni, G.
%A Busset, N.
%A Marchetti, R.
%A Palmigiano, A.
%A Moll, H.
%A Engel, R.
%A Lanzetta, R.
%A Paduano, L.
%A Parrilli, M.
%A Chang, W. S.
%A Holst, O.
%A Newman, D. K.
%A Garozzo, D.
%A D'Errico, G.
%A Giraud, Eric
%A Molinaro, A.
%T Covalently linked hopanoid-lipid A improves outer-membrane resistance of a Bradyrhizobium symbiont of legumes
%D 2014
%L fdi:010062689
%G ENG
%J Nature Communications
%@ 2041-1723
%M ISI:000343979300003
%P 5106
%R 10.1038/ncomms6106
%U https://www.documentation.ird.fr/hor/fdi:010062689
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-10/010062689.pdf
%V 5
%W Horizon (IRD)
%X Lipopolysaccharides (LPSs) are major components of the outer membrane of Gram-negative bacteria and are essential for their growth and survival. They act as a structural barrier and play an important role in the interaction with eukaryotic hosts. Here we demonstrate that a photosynthetic Bradyrhizobium strain, symbiont of Aeschynomene legumes, synthesizes a unique LPS bearing a hopanoid covalently attached to lipid A. Biophysical analyses of reconstituted liposomes indicate that this hopanoid-lipid A structure reinforces the stability and rigidity of the outer membrane. In addition, the bacterium produces other hopanoid molecules not linked to LPS. A hopanoid-deficient strain, lacking a squalene hopene cyclase, displays increased sensitivity to stressful conditions and reduced ability to survive intra-cellularly in the host plant. This unusual combination of hopanoid and LPS molecules may represent an adaptation to optimize bacterial survival in both free-living and symbiotic states.
%$ 084 ; 020