@article{fdi:010062673,
  title = {{N}ovel insights into the metabolic and biochemical underpinnings assisting dry-season survival in female malaria mosquitoes of the {A}nopheles gambiae complex},
  author = {{H}idalgo, {K}. and {M}ouline, {K}arine and {M}amai, {W}. and {F}oucreau, {N}. and {D}abire, {K}. and {B}ouchereau, {A}. and {S}imard, {F}r{\'e}d{\'e}ric and {R}enault, {D}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he mechanisms by which {A}nopheles gambiae mosquitoes survive the desiccating conditions of the dry season in {A}frica and are able to readily transmit malaria soon after the rains start remain largely unknown. {T}he desiccation tolerance and resistance of female {A}n. gambiae {M} and {S} reared in contrasting environmental conditions reflecting the onset of dry season ("ods") and the rainy season ("rs") was determined by monitoring their survival and body water loss in response to low relative humidity. {F}urthermore, we investigated the degree to which the physiology of 1-h and 24-h-old females is altered at "ods" by examining and comparing their quantitative metabotypes and proteotypes with conspecifics exposed to "rs" conditions. {R}esults showed that distinct biochemical rearrangements occurred soon after emergence in female mosquitoes that enhance survival and limit water loss under dry conditions. {I}n particular, three amino acids (phenylalanine, tyrosine, and valine) playing a pivotal role in cuticle permeability decreased significantly from the 1-h to 24-h-old females, regardless of the experimental conditions. {H}owever, these amino acids were present in higher amounts in 1-h-old female {A}n. gambiae {M} reared under "ods" whereas no such seasonal difference was reported in {S} ones. {T}ogether with the 1.28- to 2.84-fold increased expression of cuticular proteins 70 and 117, our data suggests that cuticle composition, rigidity and permeability were adjusted at "ods". {I}ncreased expression of enzymes involved in glycogenolytic and proteolytic processes were found in both forms at "ods". {M}oreover, 1-h-old {S} forms were characterised by elevated amounts of glycogen phosphorylase, isocitrate dehydrogenase, and citrate synthase, suggesting an increase of energetic demand in these females at "ods".},
  keywords = {{B}ody water ; {D}esiccation ; {M}etabolic fingerprint ; {P}roteomics ; {RR}-2 ; cuticular protein ; {T}ricarboxylic acid cycle enzyme ; {BURKINA} {FASO}},
  booktitle = {},
  journal = {{J}ournal of {I}nsect {P}hysiology},
  volume = {70},
  numero = {},
  pages = {102--116},
  ISSN = {0022-1910},
  year = {2014},
  DOI = {10.1016/j.jinsphys.2014.07.003},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062673},
}