%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Perraut, R.
%A Richard, V.
%A Varela, M. L.
%A Trape, Jean-François
%A Guillotte, M.
%A Tall, A.
%A Toure, A.
%A Sokhna, Cheikh
%A Vigan-Womas, I.
%A Mercereau-Puijalon, O.
%T Comparative analysis of IgG responses to Plasmodium falciparum MSP1p19 and PF13-DBL1 alpha 1 using ELISA and a magnetic bead-based duplex assay (MAGPIX (R)-Luminex) in a Senegalese meso-endemic community
%D 2014
%L fdi:010062657
%G ENG
%J Malaria Journal
%@ 1475-2875
%K Malaria ; Plasmodium falciparum ; ELISA ; IgG ; Surface antigens ; MSP1p19 ; PfEMP1-PF13 ; Multiplex ; MAGPIX ; Senegal ; Ndiop
%K SENEGAL
%M ISI:000344122900001
%P 410
%R 10.1186/1475-2875-13-410
%U https://www.documentation.ird.fr/hor/fdi:010062657
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-02/010062657.pdf
%V 13
%W Horizon (IRD)
%X Background: Numerous Plasmodium falciparum antigens elicit humoral responses in humans living in endemic areas. Use of multiplex assays is a convenient approach to monitor the antibody response against multiple antigens, but to integrate multiplex assay-derived data with datasets, generated previously using ELISA, comparative studies are needed. This work compares antibody responses to two P. falciparum antigens monitored using both technologies. Methods: The IgG response against the merozoite surface protein-1 PfMSP1p19 and the PF13-DBL1 alpha 1 domain of the P. falciparum Erythrocyte Membrane Protein1, expressed by the rosette-forming parasite 3D7/PF13 (PF13), was investigated using ELISA and a MAGPIX (R)-Luminex duplex assay. Archived plasma samples collected before the rainy season from 217 villagers living in Ndiop, a Senegalese meso-endemic setting, were studied. ROC analysis was used to define the optimal antibody measure readout. Association of antibody levels with protection against clinical malaria was analysed using Poisson regression in a retrospective study from active case detection records performed during the 5.5-month transmission season that followed blood sampling. Results: There was a strong positive correlation (P <10(-3)) between ELISA and MAGPIX (R)-Luminex-MFI (median fluorescence intensity) values for antibody to PfMSP1p19 (rho = 0.78) and PF13-DBL1a1 (rho = 0.89), with a similar degree of concordance in all age groups. Antibody levels to both antigens were high but displayed a different age-associated pattern. Independent age-adjusted Poisson regression analysis showed a significant association with protection only for IgG responses to MSP1p19 (P <0.01 RR = 0.71 [0.53-0.93]) measured by ELISA. Conclusion: The individual ELISA and duplex-MAGPIX assays provide a concordant evaluation of age-associated antibody responses to MSP1p19 and PF13-DBL1a1, irrespective of the formulation of antibody levels (values, ratios or ROC-adjusted figures) but do diverge with regard to the association of antibody levels with clinical protection in age-adjusted models. This may reflect incomplete overlap of the epitopes presented in the two formats. Further development for multiplex assessment of antibody responses to a larger panel of antigens with the robust and cost effective MAGPIX (R)-Luminex technology is warranted.
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