@article{fdi:010062604,
  title = {{S}enegalese sole ({S}olea senegalensis) metamorphic larvae are more sensitive to pseudo-albinism induced by high dietary arachidonic acid levels than post-metamorphic larvae},
  author = {{B}oglino, {A}. and {W}ishkerman, {A}. and {D}arias, {M}aria {J}ose and de la {I}glesia, {P}. and {A}ndree, {K}. {B}. and {G}isbert, {E}. and {E}stevez, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{H}igh dietary levels of arachidonic acid ({ARA}) and its relative proportions with eicosapentaenoic acid ({EPA}), fed during early larval stages, have been associated with malpigmentation in various flatfish species. {T}his study investigated whether the nutritional induction of pigmentary disorders at larval stages was related to a specific larval period of increased sensitivity to {ARA} in {S}enegalese sole ({S}olea senegalensis {K}aup, 1858). {S}enegalese sole larvae were fed high dietary {ARA} levels during pre- and pro-metamorphosis (2-15 dph) and/or post-metamorphosis (15-50 dph). {L}arval tissues reflected the dietary fatty acid composition. {M}alpigmentations were significantly related to elevated dietary and larval {ARA} contents and {ARA}/{EPA} ratio. {T}his study reports evidence for a "pigmentation window", with a higher larval sensitivity to dietary {ARA} during pre- and pro-metamorphosis than post-metamorphosis. {H}igh dietary {ARA} fed to larvae during pre-metamorphosis enhanced survival, but did not affect growth nor eye migration. {T}he aspect and density of melanophores in the skin of the ocular side of {ARA}-induced pseudo-albinos were significantly reduced in comparison to normally pigmented individuals, even more in the pseudo-albino fish fed high dietary {ARA} levels during the pre-metamorphic stage. {P}seudo-albino fish fed high dietary {ARA} levels during post-metamorphosis showed higher concentrations of 2- and 3-series prostaglandins ({PGE}(2) and {PGE}(3)) than normally pigmented specimens fed the same diets. {A}n increased sensitivity to {ARA}-induced malpigmentations has been identified at pre-metamorphosis and early metamorphosis in {S}enegalese sole. {S}upplying high dietary {ARA} amounts imbalanced the dietary {ARA}/{EPA} ratio and disrupted the relative concentrations of derived {PGE}(2) and {PGE}(3), resulting in 20 to 81.7% pseudo-albino individuals, depending on the dietary treatment. {T}he administration of high levels of dietary {ARA} at different developmental stages did not only affect the incidence of animals with pigmentary disorders, but it also affect the melanophore density and skin aspect in normally pigmented and pseudoalbino fish as image segmentation and texture analyses indicated.},
  keywords = {{I}mage analysis ; {F}latfish ; {L}arvae ; {P}igmentation ; {A}rachidonic acid ; {P}rostaglandins},
  booktitle = {},
  journal = {{A}quaculture},
  volume = {433},
  numero = {},
  pages = {276--287},
  ISSN = {0044-8486},
  year = {2014},
  DOI = {10.1016/j.aquaculture.2014.06.012},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062604},
}