Aberlenc Bertossi Frédérique, Castillo K., Dubreuil Tranchant Christine, Cherif E., Ballardini M., Abdoulkder S., Gros-Balthazard M., Chabrillange Nathalie, Santoni S., Mercuri A., Pintaud Jean-Christophe. (2014). In silico mining of microsatellites in coding sequences of the date palm (Arecaceae) genome, characterization, and transferability. Applications in Plant Sciences, 2 (1), art. 1300058 [5 p.]. ISSN 2168-0450.
Titre du document
In silico mining of microsatellites in coding sequences of the date palm (Arecaceae) genome, characterization, and transferability
Année de publication
2014
Auteurs
Aberlenc Bertossi Frédérique, Castillo K., Dubreuil Tranchant Christine, Cherif E., Ballardini M., Abdoulkder S., Gros-Balthazard M., Chabrillange Nathalie, Santoni S., Mercuri A., Pintaud Jean-Christophe
Source
Applications in Plant Sciences, 2014,
2 (1), art. 1300058 [5 p.] ISSN 2168-0450
Premise of the study : To complement existing sets of primarily nucleotide microsatellite loci from noncoding sequences of date palm, we developed primers for tri- and hexanueleotide microsatellite loci identified within genes. Due to their conserved genomic locations, the primers should be useful in other palm taxa, and their utility was tested in seven other Phoenix species and in Chamaerops, Liyistona, and Hyphaene. Methods and Results: Tandem repeat motifs of 3-6 bp were searched using a simple sequence repeat (SSR)-pipeline package in coding portions of the date palm draft genome sequence. Fifteen loci produced highly consistent amplification, intraspecitic polymorphisms, and stepwise mutation patterns. Conclusions: These microsatellite loci showed sufficient levels of variability and transferability to make them useful for population genetic, selection signature, and iinterspecific gene flow studies in Phoenix and other Coryphoideae genera.
Plan de classement
Sciences du monde végétal [076]
Localisation
Fonds IRD [F B010062585]
Identifiant IRD
fdi:010062585
