@article{fdi:010062580,
  title = {{M}eloidogyne incognita - rice ({O}ryza sativa) interaction : a new model system to study plant-root-knot nematode interactions in monocotyledons},
  author = {{N}guyen, {P}. {V}. and {B}ellafiore, {S}t{\'e}phane and {P}etitot, {A}nne-{S}ophie and {H}aidar, {R}. and {B}ak, {A}. and {A}bed, {A}. and {G}antet, {P}ascal and {M}ezzalira, {I}. and {E}ngler, {J}. {D}. and {F}ernandez, {D}iana},
  editor = {},
  language = {{ENG}},
  abstract = {{P}lant-parasitic nematodes developed strategies to invade and colonize their host plants, including expression of immune suppressors to overcome host defenses. {M}eloidogyne graminicola and {M}. incognita are root-knot nematode ({RKN}) species reported to damage rice ({O}ryza sativa {L}.) cultivated in upland and irrigated systems. {D}espite {M}. incognita wide host range, study of the molecular plant - {RKN} interaction has been so far limited to a few dicotyledonous model plants. {T}he aim of this study was to investigate if the rice cv. {N}ipponbare widely used in rice genomic studies could be used as a suitable monocotyledon host plant for studying {M}. incognita pathogenicity mechanisms. {H}ere we compared the ability of {M}. graminicola and {M}. incognita to develop and reproduce in {N}ipponbare roots. {N}ext, we tested if {RKN}s modulates rice immunity-related genes expression in galls during infection and express the {M}i-crt gene encoding an immune suppressor. {R}oot galling, mature females, eggs and newly formed {J}2s nematodes were obtained for both species in rice cultivated in hydroponic culture system after 4-5 weeks. {M}eloidogyne graminicola reproduced at higher rates than {M}. incognita on {N}ipponbare and the timing of infection was shorter. {I}n contrast, the infection characteristics compared by histological analysis were similar for both nematode species. {G}iant cells formed from 2 days after infection ({DAI}) with {M}. graminicola and from 6 {DAI} with {M}. incognita. {R}eal-time {PCR} (q{RT}-{PCR}) data indicated that {RKN}s are able to suppress transcription of immune regulators genes, such as {O}s{EDS}1, {O}s{PAD}4 and {O}s{WRKY}13 in young galls. {F}our {M}. incognita reference genes ({M}i-eif-3, {M}i-{GDP}-2, {M}i-{Y}45{F}10{D}.4, and {M}i-actin) were selected for normalizing nematode gene expression studies in planta and in pre-parasitic {J}2s. {M}eloidogyne incognita expressed the immune suppressor calreticulin gene ({M}i-crt) in rice roots all along its infection cycle. {RKN}s repress the transcription of key immune regulators in rice, likely in order to lower basal defence in newly-formed galls. {T}he calreticulin {M}i-{CRT} can be one of the immune-modulator effectors secreted by {M}. incognita in rice root tissues. {T}ogether, these data show that rice is a well suited model system to study host- {M}. incognita molecular interactions in monocotyledons.},
  keywords = {{R}ice ; {R}oot-knot nematodes ; {G}iant cells ; {I}mmunity suppression ; {M}eloidogyne graminicola ; {M}eloidogyne incognita},
  booktitle = {},
  journal = {{R}ice},
  volume = {7},
  numero = {},
  pages = {23 [13 ]},
  ISSN = {1939-8425},
  year = {2014},
  DOI = {10.1186/s12284-014-0023-4},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062580},
}