Publications des scientifiques de l'IRD

Perrineau MM., Le Roux C., Galiana A., Faye A., Duponnois Robin, Goh D., Prin Y., Béna Gilles. (2014). Differing courses of genetic evolution of Bradyrhizobium inoculants as revealed by long-term molecular tracing in Acacia mangium plantations. Applied and Environmental Microbiology, 80 (18), p. 5709-5716. ISSN 0099-2240.

Titre du document
Differing courses of genetic evolution of Bradyrhizobium inoculants as revealed by long-term molecular tracing in Acacia mangium plantations
Année de publication
2014
Type de document
Article référencé dans le Web of Science WOS:000341488200016
Auteurs
Perrineau MM., Le Roux C., Galiana A., Faye A., Duponnois Robin, Goh D., Prin Y., Béna Gilles
Source
Applied and Environmental Microbiology, 2014, 80 (18), p. 5709-5716 ISSN 0099-2240
Introducing nitrogen-fixing bacteria as an inoculum in association with legume crops is a common practice in agriculture. However, the question of the evolution of these introduced microorganisms remains crucial, both in terms of microbial ecology and agronomy. We explored this question by analyzing the genetic and symbiotic evolution of two Bradyrhizobium strains inoculated on Acacia mangium in Malaysia and Senegal 15 and 5 years, respectively, after their introduction. Based on typing of several loci, we showed that these two strains, although closely related and originally sampled in Australia, evolved differently. One strain was recovered in soil with the same five loci as the original isolate, whereas the symbiotic cluster of the other strain was detected with no trace of the three housekeeping genes of the original inoculum. Moreover, the nitrogen fixation efficiency was variable among these isolates (either recombinant or not), with significantly high, low, or similar efficiencies compared to the two original strains and no significant difference between recombinant and nonrecombinant isolates. These data suggested that 15 years after their introduction, nitrogen-fixing bacteria remain in the soil but that closely related inoculant strains may not evolve in the same way, either genetically or symbiotically. In a context of increasing agronomical use of microbial inoculants (for biological control, nitrogen fixation, or plant growth promotion), this result feeds the debate on the consequences associated with such practices.
Plan de classement
Biotechnologies [084]
Description Géographique
SENEGAL ; MALAISIE
Localisation
Fonds IRD [F B010062532]
Identifiant IRD
fdi:010062532
Contact