@article{fdi:010062529,
  title = {{I}dentification of continuous human {B}-cell epitopes in the {VP}35, {VP}40, nucleoprotein and glycoprotein of {E}bola virus},
  author = {{B}ecquart, {P}ierre and {M}ahlakoiv, {T}. and {N}koghe, {D}. and {L}eroy, {E}ric},
  editor = {},
  language = {{ENG}},
  abstract = {{E}bola virus ({EBOV}) is a highly virulent human pathogen. {R}ecovery of infected patients is associated with efficient {EBOV}-specific immunoglobulin {G} ({I}g{G}) responses, whereas fatal outcome is associated with defective humoral immunity. {A}s {B}-cell epitopes on {EBOV} are poorly defined, we sought to identify specific epitopes in four {EBOV} proteins ({G}lycoprotein ({GP}), {N}ucleoprotein ({NP}), and matrix {V}iral {P}rotein ({VP})40 and {VP}35). {F}or the first time, we tested {EBOV} {I}g{G}+ sera from asymptomatic individuals and symptomatic {G}abonese survivors, collected during the early humoral response (seven days after the end of symptoms) and the late memory phase (7-12 years post-infection). {W}e also tested sera from {EBOV}-seropositive patients who had never had clinical signs of hemorrhagic fever or who lived in non-epidemic areas (asymptomatic subjects). {W}e found that serum from asymptomatic individuals was more strongly reactive to {VP}40 peptides than to {GP}, {NP} or {VP}35. {I}nterestingly, anti-{EBOV} {I}g{G} from asymptomatic patients targeted three immunodominant regions of {VP}40 reported to play a crucial role in virus assembly and budding. {I}n contrast, serum from most survivors of the three outbreaks, collected a few days after the end of symptoms, reacted mainly with {GP} peptides. {H}owever, in asymptomatic subjects the longest immunodominant domains were identified in {GP}, and analysis of the {GP} crystal structure revealed that these domains covered a larger surface area of the chalice bowl formed by three {GP}(1) subunits. {T}he {B}-cell epitopes we identified in the {EBOV} {VP}35, {VP}40, {NP} and {GP} proteins may represent important tools for understanding the humoral response to this virus and for developing new antibody-based therapeutics or detection methods.},
  keywords = {{GABON}},
  booktitle = {},
  journal = {{PL}o{S} {O}ne},
  volume = {9},
  numero = {6},
  pages = {e96360},
  ISSN = {1932-6203},
  year = {2014},
  DOI = {10.1371/journal.pone.0096360},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062529},
}