@article{fdi:010062528,
  title = {{C}omparative assessment of the bacterial communities associated with {A}edes aegypti larvae and water from domestic water storage containers},
  author = {{D}ada, {N}. and {J}umas-{B}ilak, {E}. and {M}anguin, {S}ylvie and {S}eidu, {R}. and {S}tenstrom, {T}. {A}. and {O}vergaard, {H}. {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {D}omestic water storage containers constitute major {A}edes aegypti breeding sites. {W}e present for the first time a comparative analysis of the bacterial communities associated with {A}e. aegypti larvae and water from domestic water containers. {M}ethods: {T}he 16{S} r{RNA}-temporal temperature gradient gel electrophoresis ({TTGE}) was used to identify and compare bacterial communities in fourth-instar {A}e. aegypti larvae and water from larvae positive and negative domestic containers in a rural village in northeastern {T}hailand. {W}ater samples were cultured for enteric bacteria in addition to {TTGE}. {S}equences obtained from {TTGE} and bacterial cultures were clustered into operational taxonomic units ({OTU}s) for analyses. {R}esults: {S}ignificantly lower {OTU} abundance was found in fourth-instar {A}e. aegypti larvae compared to mosquito positive water samples. {T}here was no significant difference in {OTU} abundance between larvae and mosquito negative water samples or between mosquito positive and negative water samples. {L}arval samples had significantly different {OTU} diversity compared to mosquito positive and negative water samples, with no significant difference between mosquito positive and negative water samples. {T}he {TTGE} identified 24 bacterial taxa, belonging to the phyla {P}roteobacteria, {F}irmicutes, {A}ctinobacteria, {B}acteroidetes and {TM}7 (candidate phylum). {S}even of these taxa were identified in larval samples, 16 in mosquito positive and 13 in mosquito negative water samples. {O}nly two taxa, belonging to the phyla {F}irmicutes and {A}ctinobacteria, were common to both larvae and water samples. {B}acilli was the most abundant bacterial class identified from {A}e. aegypti larvae, {G}ammaproteobacteria from mosquito positive water samples, and {F}lavobacteria from mosquito negative water samples. {E}nteric bacteria belonging to the class {G}ammaproteobacteria were sparsely represented in {TTGE}, but were isolated from both mosquito positive and negative water samples by selective culture. {C}onclusions: {F}ew bacteria from water samples were identified in fourth-instar {A}e. aegypti larvae, suggesting that established larval bacteria, most likely acquired at earlier stages of development, control the larval microbiota. {F}urther studies at all larval stages are needed to fully understand the dynamics involved. {I}solation of enteric bacteria from water samples supports earlier outcomes of {E}. coli contamination in {A}e. aegypti infested domestic containers, suggesting the need to further explore the role of enteric bacteria in {A}e. aegypti infestation.},
  keywords = {{A}edes aegypti ; {B}acterial diversity ; {E}nteric bacteria ; {E}. coli ; {D}omestic ; water storage containers ; 16{S} r{RNA}-{TTGE} ; {T}hailand ; {THAILANDE}},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {7},
  numero = {},
  pages = {art. 391 [12 ]},
  ISSN = {1756-3305},
  year = {2014},
  DOI = {10.1186/1756-3305-7-391},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062528},
}