@article{fdi:010062499,
  title = {{E}vidence of dengue virus transmission and factors associated with the presence of anti-dengue virus antibodies in humans in three major towns in {C}ameroon},
  author = {{D}emanou, {M}. and {P}ouillot, {R}. and {G}randadam, {M}. and {B}oisier, {P}. and {K}amgang, {B}. and {H}erv{\'e}, {J}ean-{P}ierre and {R}ogier, {C}. and {R}ousset, {D}. and {P}aupy, {C}hristophe},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {D}engue is not well documented in {A}frica. {I}n {C}ameroon, data are scarce, but dengue infection has been confirmed in humans. {W}e conducted a study to document risk factors associated with anti-dengue virus {I}mmunoglobulin {G} seropositivity in humans in three major towns in {C}ameroon. {M}ethodology/{P}rincipal {F}indings: {A} cross sectional survey was conducted in {D}ouala, {G}aroua and {Y}aounde, using a random cluster sampling design. {P}articipants underwent a standardized interview and were blood sampled. {E}nvironmental and housing characteristics were recorded. {R}andomized houses were prospected to record all water containers, and immature stages of {A}edes mosquitoes were collected. {S}era were screened for anti-dengue virus {I}g{G} and {I}g{M} antibodies. {R}isk factors of seropositivity were tested using logistic regression methods with random effects. {A}nti-dengue {I}g{G} were found from 61.4% of sera in {D}ouala (n = 699), 24.2% in {G}aroua (n = 728) and 9.8% in {Y}aounde (n = 603). {I}g{M} were found from 0.3% of {D}ouala samples, 0.1% of {G}aroua samples and 0.0% of {Y}aounde samples. {S}eroneutralization on randomly selected {I}g{G} positive sera showed that 72% (n = 100) in {D}ouala, 80% (n = 94) in {G}aroua and 77% (n = 66) in {Y}aounde had antibodies specific for dengue virus serotype 2 ({DENV}-2). {A}ge, temporary house walls materials, having water-storage containers, old tires or toilets in the yard, having no {TV}, having no air conditioning and having travelled at least once outside the city were independently associated with anti-dengue {I}g{G} positivity in {D}ouala. {A}ge, having uncovered water containers, having no {TV}, not being born in {G}aroua and not breeding pigs were significant risk factors in {G}aroua. {R}ecent history of malaria, having banana trees and stagnant water in the yard were independent risk factors in {Y}aounde. {C}onclusion/{S}ignificance: {I}n this survey, most identified risk factors of dengue were related to housing conditions. {P}overty and underdevelopment are central to the dengue epidemiology in {C}ameroon.},
  keywords = {{CAMEROUN}},
  booktitle = {},
  journal = {{P}los {N}eglected {T}ropical {D}iseases},
  volume = {8},
  numero = {7},
  pages = {e2950},
  ISSN = {1935-2735},
  year = {2014},
  DOI = {10.1371/journal.pntd.0002950},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062499},
}