Publications des scientifiques de l'IRD

Markovic Z., Preiner D., Bosnjak A. M., Safner T., Stupic D., Andabaka Z., Maletic E., Chatelet P., Engelmann Florent, Kontic J. K. (2014). In vitro introduction of healthy and virus-infected genotypes of native Croatian grapevine cultivars. Central European Journal of Biology, 9 (11), p. 1087-1098. ISSN 1895-104X.

Titre du document
In vitro introduction of healthy and virus-infected genotypes of native Croatian grapevine cultivars
Année de publication
2014
Type de document
Article référencé dans le Web of Science WOS:000340665500009
Auteurs
Markovic Z., Preiner D., Bosnjak A. M., Safner T., Stupic D., Andabaka Z., Maletic E., Chatelet P., Engelmann Florent, Kontic J. K.
Source
Central European Journal of Biology, 2014, 9 (11), p. 1087-1098 ISSN 1895-104X
We evaluated the response of eight economically important Croatian grapevine cultivars and studied the impact of their sanitary status on in vitro introduction, by comparing the response of healthy and virus-infected genotypes of one cultivar. Nodal explant survival on three media, M1 (half-strength MS), M2 (full-strength MS) or M3 (full-strength MS with 4.4 A mu M L-1 benzylaminopurine) was measured after 2 weeks and regrowth after 8 weeks. After 8 weeks, average shoot length and node number were significantly higher on M2 compared to M1 and M3. M3 induced significantly shorter average internode length, compared to M1 and M2. Survival of one healthy and of five cultivar Plavac mali genotypes infected with GFLV, GLRaV-1, GLRaV-3, GLRaV-3+GVA and GLRaV-1+GLRaV-3 was 97.5 and 82.8-87.5%, respectively. Regrowth of the healthy genotype reached 95.5%, but dropped to 5.5-31.4% in infected ones. The healthy genotype showed significantly higher shoot length (6.3 cm) and node number (7.3) compared to infected genotypes, with shoot length between 1.2-2.6 cm and node number between 1.2-3.0. By contrast, internode length was not significantly different between the healthy and the infected genotypes. The present work represents the first successful in vitro introduction for three of the eight native Croatian cultivars studied.
Plan de classement
Sciences du monde végétal [076] ; Biotechnologies [084]
Description Géographique
CROATIE
Localisation
Fonds IRD [F B010062480]
Identifiant IRD
fdi:010062480
Contact