%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Sharakhova, M. V.
%A Antonio-Nkondjio, C.
%A Xia, A.
%A Ndo, C.
%A Awono-Ambene, P.
%A Simard, Frédéric
%A Sharakhov, I. V.
%T Polymorphic chromosomal inversions in Anopheles moucheti, a major malaria vector in Central Africa
%D 2014
%L fdi:010062469
%G ENG
%J Medical and Veterinary Entomology
%@ 0269-283X
%K Anopheles gambiae ; Anopheles moucheti ; fluorescent in situ ; hybridization inversion ; malaria mosquito ; physical mapping ; polytene ; chromosome
%K CAMEROUN SUD ; AFRIQUE CENTRALE
%M ISI:000340420500013
%N 3
%P 337-340
%R 10.1111/mve.12037
%U https://www.documentation.ird.fr/hor/fdi:010062469
%> https://www.documentation.ird.fr/intranet/publi/2014/09/010062469.pdf
%V 28
%W Horizon (IRD)
%X Anopheles moucheti Evans (Diptera: Culicidae) is a major vector of malaria in forested areas of Central Africa. However, few genetic tools are available for this species. The present study represents the first attempt to characterize chromosomes in An. moucheti females collected in Cameroon. Ovarian nurse cells contained polytene chromosomes, which were suitable for standard cytogenetic applications. The presence of three polymorphic chromosomal inversions in An. moucheti was revealed. Two of these inversions were located on the 2R chromosome arm. The homology between the 2R chromosome arms of An. moucheti and Anopheles gambiae Giles was established by fluorescent in situ hybridization of six An. gambiae genic sequences. Mapping of the probes on chromosomes of An. moucheti detected substantial gene order reshuffling between the two species. The presence of polytene chromosomes and polymorphic inversions in An. moucheti provides a new basis for further population genetic, taxonomic and ecological studies of this neglected malaria vector.
%$ 052