@article{fdi:010062429,
  title = {{A}ssociation of antibody responses to the conserved {P}lasmodium falciparum merozoite surface protein 5 with protection against clinical malaria},
  author = {{P}erraut, {R}. and {J}oos, {C}. and {S}okhna, {C}heikh and {P}olson, {H}. {E}. {J}. and {T}rape, {J}ean-{F}ran{\c{c}}ois and {T}all, {A}. and {M}arrama, {L}. and {M}ercereau-{P}uijalon, {O}. and {R}ichard, {V}. and {L}ongacre, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {P}lasmodium falciparum merozoite surface protein 5 ({P}f{MSP}5) is an attractive blood stage vaccine candidate because it is both exposed to the immune system and well conserved. {T}o evaluate its interest, we investigated the association of anti-{P}f{MSP}5 {I}g{G} levels, in the context of responses to two other conserved {A}gs {P}f{MSP}1p19 and {R}23, with protection from clinical episodes of malaria in cross-sectional prospective studies in two different transmission settings. {M}ethods: {N}diop (mesoendemic) and {D}ielmo (holoendemic) are two {S}enegalese villages participating in an on-going long-term observational study of natural immunity to malaria. {B}lood samples were taken before the transmission season ({N}diop) or before peak transmission ({D}ielmo) and active clinical surveillance was carried out during the ensuing 5.5-month follow-up. {I}g{G} responses to recombinant {P}f{MSP}5, {P}f{MSP}1p19 and {R}23 were quantified by {ELISA} in samples from surveys carried out in {D}ielmo (186 subjects) and {N}diop (221 subjects) in 2002, and {N}diop in 2000 (204 subjects). {I}n addition, 236 sera from the {D}ielmo and {N}diop-2002 surveys were analyzed for relationships between the magnitude of anti-{P}f{MSP}5 response and neutrophil antibody dependent respiratory burst ({ADRB}) activity. {R}esults: {A}nti-{P}f{MSP}5 antibodies predominantly {I}g{G}1 were detected in 60-74% of villagers, with generally higher levels in older age groups. {P}f{MSP}5 {I}g{G} responses were relatively stable for {N}diop subjects sampled both in 2000 and 2002. {ADRB} activity correlated with age and anti-{P}f{MSP}5 {I}g{G} levels. {I}mportantly, {P}f{MSP}5 antibody levels were significantly associated with reduced incidence of clinical malaria in all three cohorts. {I}nclusion of {I}g{G} to {P}f{MSP}1p19 in the poisson regression model did not substantially modify results. {C}onclusion: {T}hese results indicate that {MSP}5 is recognized by naturally acquired {A}b. {T}he large seroprevalence and association with protection against clinical malaria in two settings with differing transmission conditions and stability over time demonstrated in {N}diop argue for further evaluation of baculovirus {P}f{MSP}5 as a vaccine candidate.},
  keywords = {{SENEGAL}},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {9},
  numero = {7},
  pages = {e101737},
  ISSN = {1932-6203},
  year = {2014},
  DOI = {10.1371/journal.pone.0101737},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062429},
}