@article{fdi:010062419,
  title = {{D}etermination of expression and activity of genes involved in starch metabolism in {L}actobacillus plantarum {A}6 during fermentation of a cereal-based gruel},
  author = {{H}umblot, {C}hrist{\`e}le and {T}urpin, {W}. and {C}hevalier, {F}. and {P}icq, {C}hristian and {R}ochette, {I}sabelle and {G}uyot, {J}ean-{P}ierre},
  editor = {},
  language = {{ENG}},
  abstract = {{T}raditional fermented gruels prepared from cereals are widely used for complementary feeding of young children in {A}frica and usually have a low energy density. {T}he amylase activity of some lactic acid bacteria ({LAB}) helps increase the energy content of gruels through partial hydrolysis of starch, thus enabling the incorporation of more starchy material while conserving the desired semi-liquid consistency for young children. {E}ven if this ability is mainly related to the production of alpha-amylase ({E}.{C}. 3.2.1.1), in a recent molecular screening, genes coding for enzymes involved in starch metabolism were detected in the efficient amylolytic {LAB} {L}actobacillus plantarum {A}6: alpha-glucosidase ({E}.{C}. 32.1.20), neopullulanase ({E}.{C} 3.2.1.135), amylopectin phosphorylase ({E}.{C}. 2.4.1.1) and maltose phosphorylase ({E}.{C}. 2.4.1.8). {T}he objective of this study was to investigate the expression of these genes in a model of starchy fermented food made from pearl millet ({P}ennisetum glaucum). {T}ranscriptional and enzymatic analyses were performed during the 18-h fermentation period. {L}iquefaction was mainly caused by an extracellular alpha amylase encoded by the amy{A} gene specific to the {A}6 strain among {L}. plantarum species and found in both {L}actobacillus amylovorus and {L}actobacillus manihotivorans. {T}he second most active enzyme was neopullulanase. {O}ther starch metabolizing enzymes were less often detected. {T}he dynamic detection of transcripts of gene during starch fermentation in pearl millet porridge suggests that the set of genes we investigated was not expressed continuously but transiently.},
  keywords = {m{RNA} ; {R}eal time {PCR} ; {P}earl millet ; {K}inetics ; {BURKINA} {FASO} ; {GHANA} ; {AFRIQUE} {SUBSAHARIENNE}},
  booktitle = {},
  journal = {{I}nternational {J}ournal of {F}ood {M}icrobiology},
  volume = {185},
  numero = {},
  pages = {103--111},
  ISSN = {0168-1605},
  year = {2014},
  DOI = {10.1016/j.ijfoodmicro.2014.05.016},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062419},
}