@article{fdi:010062393,
  title = {{A}lternative mutational pathways, outside the {VP}g, of rice yellow mottle virus to overcome e{IF}(iso)4{G}-mediated rice resistance under strong genetic constraints},
  author = {{P}oulicard, {N}. and {G}alzi, {A}gn{\`e}s and {F}argette, {D}enis and {H}{\'e}brard, {E}ug{\'e}nie},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he adaptation of rice yellow mottle virus ({RYMV}) to rymv1-mediated resistance has been reported to involve mutations in the viral genome-linked protein ({VP}g). {I}n this study, we analysed several cases of rymv1-2 resistance breakdown by an isolate with low adaptability. {S}urprisingly, in these rarely occurring resistance-breaking ({RB}) genotypes, mutations were detected outside the {VP}g, in the {ORF}2a/{ORF}2b overlapping region. {T}he causal role of three mutations associated with rymv1-2 resistance breakdown was validated via directed mutagenesis of an infectious clone. {I}n resistant plants, these mutations increased viral accumulation as efficiently as suboptimal {RB} mutations in the {VP}g. {I}nterestingly, these mutations are located in a highly conserved, but unfolded, domain. {A}ltogether, our results indicate that under strong genetic constraints, a priori unfit genotypes can follow alternative mutational pathways, i.e. outside the {VP}g, to overcome rymv1-2 resistance.},
  keywords = {},
  booktitle = {},
  journal = {{J}ournal of {G}eneral {V}irology},
  volume = {95},
  numero = {1},
  pages = {219--224},
  ISSN = {0022-1317},
  year = {2014},
  DOI = {10.1099/vir.0.057810-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062393},
}