@article{fdi:010062383,
  title = {{A} quality control program within a clinical trial consortium for {PCR} protocols to detect {P}lasmodium species},
  author = {{T}aylor, {S}. {M}. and {M}ayor, {A}. and {M}ombo-{N}goma, {G}. and {K}enguele, {H}. {M}. and {O}uedraogo, {S}. and {T}uikue {N}dam, {N}icaise and {M}kali, {H}. and {M}wangoka, {G}. and {V}alecha, {N}. and {S}ingh, {J}. {P}. {N}. and {C}lark, {M}. {A}. and {V}erweij, {J}. {J}. and {A}degnika, {A}. {A}. and {S}everini, {C}. and {M}enegon, {M}. and {M}acete, {E}. and {M}enendez, {C}. and {C}istero, {P}. and {N}jie, {F}. and {A}ffara, {M}. and {O}tieno, {K}. and {K}ariuki, {S}. and ter {K}uile, {F}. {O}. and {M}eshnick, {S}. {R}.},
  editor = {},
  language = {{ENG}},
  abstract = {{M}alaria parasite infections that are only detectable by molecular methods are highly prevalent and represent a potential transmission reservoir. {T}he methods used to detect these infections are not standardized, and their operating characteristics are often unknown. {W}e designed a proficiency panel of {P}lasmodium spp. in order to compare the accuracy of parasite detection of molecular protocols used by labs in a clinical trial consortium. {T}en dried blood spots ({DBS}s) were assembled that contained {P}. falciparum, {P}. vivax, {P}. malariae, and {P}. ovale; {DBS}s contained either a single species or a species mixed with {P}. falciparum. {DBS} panels were tested in 9 participating laboratories in a masked fashion. {O}f 90 tests, 68 (75.6%) were correct; there were 20 false-negative results and 2 false positives. {T}he detection rate was 77.8% (49/63) for {P}. falciparum, 91.7% (11/12) for {P}. vivax, 83.3% (10/12) for {P}. malariae, and 70% (7/10) for {P}. ovale. {M}ost false-negative {P}. falciparum results were from samples with an estimated <= 5 parasites per mu l of blood. {B}etween labs, accuracy ranged from 100% to 50%. {I}n one lab, the inability to detect species in mixed-species infections prompted a redesign and improvement of the assay. {M}ost {PCR}-based protocols were able to detect {P}. falciparum and {P}. vivax at higher densities, but these assays may not reliably detect parasites in samples with low {P}. falciparum densities. {A}ccordingly, formal quality assurance for {PCR} should be employed whenever this method is used for diagnosis or surveillance. {S}uch efforts will be important if {PCR} is to be widely employed to assist malaria elimination efforts.},
  keywords = {},
  booktitle = {},
  journal = {{J}ournal of {C}linical {M}icrobiology},
  volume = {52},
  numero = {6},
  pages = {2144--2149},
  ISSN = {0095-1137},
  year = {2014},
  DOI = {10.1128/jcm.00565-14},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062383},
}