@article{fdi:010062381,
  title = {{D}evelopment of a genomics-based {LAMP} ({L}oop-{M}ediated {I}sothermal {A}mplification) assay for detection of {P}seudomonas fuscovaginae from rice},
  author = {{A}sh, {G}.{J}. and {L}ang, {J}.{M}. and {T}riplett, {L}.{R}. and {S}todart, {B}.{J}. and {V}erdier, {V}al{\'e}rie and {C}ruz, {C}.{V}. and {R}ott, {P}. and {L}each, {J}. {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he vast amount of data available through next-generation sequencing technology is facilitating the design of diagnostic marker systems. {T}his study reports the use of draft genome sequences from the bacterial plant pathogen {P}seudomonas fuscovaginae, the cause of sheath brown rot of rice, to describe the genetic diversity within a worldwide collection of strains representing the species. {B}ased on a comparative analysis with the draft sequences, primers for a loop-mediated isothermal amplification ({LAMP}) assay were developed to identify {P}. fuscovaginae. {T}he assay reported here reliably differentiated strains of {P}. fuscovaginae isolated from rice from a range of other bacteria that are commonly isolated from rice and other plants using a primer combination designated {P}f8. {T}he {LAMP} assay identified {P}. fuscovaginae purified {DNA}, live or heat-killed cells from pure cultures, and detected the bacterium in extracts or exudates from infected host plant material. {T}he {P}. fuscovaginae {LAMP} assay is a suitable diagnostic tool for the glasshouse and laboratory and could be further developed for in-field surveys.},
  keywords = {},
  booktitle = {},
  journal = {{P}lant {D}isease},
  volume = {98},
  numero = {7},
  pages = {909--915},
  ISSN = {0191-2917},
  year = {2014},
  DOI = {10.1094/pdis-09-13-0957-re},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062381},
}