@article{fdi:010062373,
  title = {{G}enotypic and symbiotic diversity of {R}hizobium populations associated with cultivated lentil and pea in sub-humid and semi-arid regions of {E}astern {A}lgeria},
  author = {{R}iah, {N}. and {B}{\'e}na, {G}illes and {D}jekoun, {A}. and {H}eulin, {K}. and {L}ajudie de, {P}hilippe and {L}aguerre, {G}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he genetic structure of rhizobia nodulating pea and lentil in {A}lgeria, {N}orthern {A}frica was determined. {A} total of 237 isolates were obtained from root nodules collected on lentil ({L}ens culinaris), proteaginous and forage pea ({P}isum sativum) growing in two eco-climatic zones, sub-humid and semi-arid, in {E}astern {A}lgeria. {T}hey were characterised by {PCR}-restriction fragment length polymorphism ({RFLP}) of the 16{S}-23{S} r{RNA} intergenic region ({IGS}), and the nod{D}-{F} symbiotic region. {T}he combination of these haplotypes allowed the isolates to be clustered into 26 distinct genotypes, and all isolates were classified as {R}hizobium leguminosarum. {S}ymbiotic marker variation (nod{D}-{F}) was low but with the predominance of one nod haplotype (g), which had been recovered previously at a high frequency in {E}urope. {S}equence analysis of the {IGS} further confirmed its high variability in the studied strains. {A}n {AMOVA} analysis showed highly significant differentiation in the {IGS} haplotype distribution between populations from both eco-climatic zones. {T}his differentiation was reflected by differences in dominant genotype frequencies. {C}onversely, no host plant effect was detected. {T}he nod{D} gene sequence-based phylogeny suggested that symbiotic gene diversity in pea and lentil nodulating rhizobial populations in {A}lgeria was low compared to that reported elsewhere in the world.},
  keywords = {{L}entil ; {P}ea ; {R}hizobium ; {A}lgeria ; {P}opulation genetic structure ; {E}co-climatic zones ; {ALGERIE}},
  booktitle = {},
  journal = {{S}ystematic and {A}pplied {M}icrobiology},
  volume = {37},
  numero = {5},
  pages = {368--375},
  ISSN = {0723-2020},
  year = {2014},
  DOI = {10.1016/j.syapm.2013.12.008},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062373},
}