Publications des scientifiques de l'IRD

Pruvost O., Magne M., Boyer K., Leduc A., Tourterel C., Drevet C., Ravigné V., Gagnevin L., Guérin F., Chiroleu F., Koebnik Ralf, Verdier Valérie, Vernière C. (2014). A MLVA genotyping scheme for global surveillance of the Citrus pathogen Xanthomonas citri pv. citri suggests a worldwide geographical expansion of a single genetic lineage. Plos One, 9 (6), e98129. ISSN 1932-6203.

Titre du document
A MLVA genotyping scheme for global surveillance of the Citrus pathogen Xanthomonas citri pv. citri suggests a worldwide geographical expansion of a single genetic lineage
Année de publication
2014
Type de document
Article référencé dans le Web of Science WOS:000338430700026
Auteurs
Pruvost O., Magne M., Boyer K., Leduc A., Tourterel C., Drevet C., Ravigné V., Gagnevin L., Guérin F., Chiroleu F., Koebnik Ralf, Verdier Valérie, Vernière C.
Source
Plos One, 2014, 9 (6), e98129 ISSN 1932-6203
MultiLocus Variable number of tandem repeat Analysis (MLVA) has been extensively used to examine epidemiological and evolutionary issues on monomorphic human pathogenic bacteria, but not on bacterial plant pathogens of agricultural importance albeit such tools would improve our understanding of their epidemiology, as well as of the history of epidemics on a global scale. Xanthomonas citri pv. citri is a quarantine organism in several countries and a major threat for the citrus industry worldwide. We screened the genomes of Xanthomonas citri pv. citri strain IAPAR 306 and of phylogenetically related xanthomonads for tandem repeats. From these in silico data, an optimized MLVA scheme was developed to assess the global diversity of this monomorphic bacterium. Thirty-one minisatellite loci (MLVA-31) were selected to assess the genetic structure of 129 strains representative of the worldwide pathological and genetic diversity of X. citri pv. citri. Based on Discriminant Analysis of Principal Components (DAPC), four pathotype-specific clusters were defined. DAPC cluster 1 comprised strains that were implicated in the major geographical expansion of X. citri pv. citri during the 20th century. A subset of 12 loci (MLVA-12) resolved 89% of the total diversity and matched the genetic structure revealed by MLVA-31. MLVA-12 is proposed for routine epidemiological identification of X. citri pv. citri, whereas MLVA-31 is proposed for phylogenetic and population genetics studies. MLVA-31 represents an opportunity for international X. citri pv. citri genotyping and data sharing. The MLVA-31 data generated in this study was deposited in the Xanthomonas citri genotyping database (http://www.biopred.net/MLVA/).
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020] ; Sciences du monde végétal [076] ; Biotechnologies [084]
Localisation
Fonds IRD [F B010062370]
Identifiant IRD
fdi:010062370
Contact