@article{fdi:010062228,
  title = {{S}ensitivity and specificity of {HAT} {S}ero-{K}-{S}e{T}, a rapid diagnostic test for serodiagnosis of sleeping sickness caused by {T}rypanosoma brucei gambiense : a case-control study},
  author = {{B}uscher, {P}. and {M}ertens, {P}. and {L}eclipteux, {T}. and {G}illeman, {Q}. and {J}acquet, {D}. and {M}umba-{N}goyi, {D}. and {P}yana, {P}. {P}. and {B}oelaert, {M}. and {L}ejon, {V}eerle},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {H}uman {A}frican trypanosomiasis ({HAT}) is a life-threatening infection affecting rural populations in sub-{S}aharan {A}frica. {L}arge-scale population screening by antibody detection with the {C}ard {A}gglutination {T}est for {T}rypanosomiasis ({CATT})/{T}rypanosoma brucei ({T} b) gambiense helped reduce the number of reported cases of gambiense {HAT} to fewer than 10 000 in 2011. {B}ecause low case numbers lead to decreased cost-effectiveness of such active screening, we aimed to assess diagnostic accuracy of a rapid serodiagnostic test ({HAT} {S}ero-{K}-{S}e{T}) applicable in primary health-care centres. {M}ethods {I}n our case-control study, we assessed participants older than 11 years who presented for {HAT} {S}ero-{K}-{S}e{T} and {CATT}/{T} b gambiense at primary care centres or to mobile teams (and existing patients with confirmed disease status at these centres) in {B}andundu {P}rovince, {DR} {C}ongo. {W}e defined cases as patients with trypanosomes that had been identified in lymph node aspirate, blood, or cerebrospinal fluid. {D}uring screening, we recruited controls without previous history of {HAT} or detectable trypanosomes in blood or lymph who resided in the same area as the cases. {W}e assessed diagnostic accuracy of three antibody detection tests for gambiense {HAT}: {HAT} {S}ero-{K}-{S}e{T} and {CATT}/{T} b gambiense (done with venous blood at the primary care centres) and immune trypanolysis (done with plasma at the {I}nstitute of {T}ropical {M}edicine, {A}ntwerp, {B}elgium). {F}indings {B}etween {J}une 6, 2012, and {F}eb 25, 2013, we included 134 cases and 356 controls. {HAT} {S}ero-{K}-{S}e{T} had a sensitivity of 0.985 (132 true positives, 95% {CI} 0.947-0.996) and a specificity of 0.986 (351 true negatives, 0.968-0.994), which did not differ significantly from {CATT}/{T} b gambiense (sensitivity 95% {CI} 0.955, 95% {CI} 0.906-0.979 [128 true positives] and specifi city 0.972, 0.949-0.985 [346 true negatives]) or immune trypanolysis (sensitivity 0.985, 0.947-0.996 [132 true positives] and specifi city 0.980, 0.960-0.990 [349 true negatives]). {I}nterpretation {T}he diagnostic accuracy of {HAT} {S}ero-{K}-{S}e{T} is adequate for {T} b gambiense antibody detection in local health centres and could be used for active screening whenever a cold chain and electricity supply are unavailable and {CATT}/{T} b gambiense cannot be done. {F}unding {E}uropean {C}ommission {FP}7 ({NIDIAG}, {G}rant {A}greement 260260) and {WHO} {C}ontrol of {N}eglected {T}ropical {D}iseases, {I}nnovative and {I}ntensified {D}isease {M}anagement.},
  keywords = {{REPUBLIQUE} {DEMOCRATIQUE} {DU} {CONGO}},
  booktitle = {},
  journal = {{L}ancet {G}lobal {H}ealth},
  volume = {2},
  numero = {6},
  pages = {{E}359--{E}363},
  ISSN = {2214-109{X}},
  year = {2014},
  DOI = {10.1016/s2214-109x(14)70203-7},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062228},
}