@article{fdi:010062153,
  title = {{C}ad{A} of {M}esorhizobium metallidurans isolated from a zinc-rich mining soil is a {P}({IB}-2)-type {ATP}ase involved in cadmium and zinc resistance},
  author = {{M}aynaud, {G}. and {B}runel, {B}. and {Y}ashiro, {E}. and {M}ergeay, {M}. and {C}leyet-{M}arel, {J}.{C}. and {L}e {Q}u{\'e}r{\'e}, {A}ntoine},
  editor = {},
  language = {{ENG}},
  abstract = {{M}esorhizobium metallidurans {STM} 2683({T}) is a nitrogen-fixing bacterium that nodulates {A}nthyllis vulneraria in mine tailings highly contaminated in zinc, lead and cadmium. {T}o study the mechanisms whereby this bacterium copes with metals, we functionally screened a cosmid genomic library of {M}. metallidurans for zinc or cadmium tolerance. {A} cosmid clone harbored a gene encoding {P}({IB})-type {ATP}ase homologous to {C}ad{A} that leads to cadmium and zinc resistance in {E}scherichia coli. {T}he {C}ad{A} protein structure presents one duplication of the two {N}-terminal metal binding domains (i.e. a heavy metal-associated domain followed by a histidine-rich domain) which allows specific binding to zinc and cadmium cations. {A} cad{A}-deleted strain of {M}. metallidurans failed to grow at high zinc concentrations (2 m{M}) and its growth was delayed at lower zinc concentrations. {E}xpression studies using a transcriptional fusion of cad{A} promoter to gfp showed that cad{A} is specifically induced in a dose-dependent manner by zinc and cadmium in {M}. metallidurans in vitro conditions and into {A}. vulneraria nodules after {Z}n stress. {M}etal induction sensitivity was increased in the strain where cad{A} gene was deleted. {T}his study identified cad{A} as a first mesorhizobial resistance determinant involved in detoxification of cadmium and zinc and which confers upon {M}. metallidurans greater capacity for coping with high zinc concentrations. {T}his improves the knowledge of this bacterium for potential use as a symbiotic inoculant of {A}nthyllis in phytostabilization strategies of metal-rich sites.},
  keywords = {{BACTERIE} ; {FIXATION} {BIOLOGIQUE} {DE} {L}'{AZOTE} ; {LEGUMINEUSE} ; {METAL} ; {ZINC} ; {PLOMB} ; {TOLERANCE} ; {RESISTANCE} ; {MINE} ; {POLLUTION} ; {BIOLOGIE} {MOLECULAIRE} ; {GENE} ; {PHYLOGENIE} ; {MUTATION} ; {ETUDE} {EXPERIMENTALE} ; {ETUDE} {COMPARATIVE} ; {CADMIUM} ; {TECHNIQUE} {PCR} ; {GENOME} ; {FRANCE} ; {SAINT} {LAURENT} {LE} {MINIER}},
  booktitle = {},
  journal = {{R}esearch in {M}icrobiology},
  volume = {165},
  numero = {3},
  pages = {175--189},
  ISSN = {1769-7123},
  year = {2014},
  DOI = {10.1016/j.resmic.2014.02.001},
  URL = {https://www.documentation.ird.fr/hor/fdi:010062153},
}