@article{fdi:010061990,
  title = {{D}ried blood spots for {HIV}-1 drug resistance genotyping in decentralized settings in {S}enegal},
  author = {{D}iouara, {A}. {M}. and {D}iop-{N}diaye, {H}. and {K}ebe-{F}all, {K}. and {T}chiakpe, {E}. and {N}diaye, {O}. and {A}youba, {A}hidjo and {P}eeters, {M}artine and {M}boup, {S}. and {K}ane, {C}. {T}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he aim of this study was to evaluate the use for {HIV}-1 drug resistance testing dried blood spots collected in remote areas and sent under field conditions to a reference laboratory and also to document virological failure in patients with suspected treatment failure. {S}amples were collected from patients receiving first line {ART} at 11 hospital sites around country, kept at room temperature (<37 degrees {C}) and sent within 15 days maximum to the reference laboratory. {V}iral nucleic acids were obtained by magnetic extraction with {N}ucli{SENS} (bio{M}erieux, {M}arcy l'{E}toile, {F}rance). {G}enotyping of {HIV}-1 pol gene was performed using the {ANRS} protocol. {D}rug resistance mutations were analyzed according to the {S}tanford {U}niversity {HIV} database version 6.0.8. {T}wo hundred thirty one {HIV}-infected adults' on {HAART} first line regimen composed study population. {T}he median time on {ART} was 18 months (range 6-68). {R}egardless of the treatment duration, the overall rate of virological failure ({VL} >= 3 log(10) cp/ml) was 23.8% (n = 55/231). {HIV} genotypes were obtained successfully in 94.5% (n = 52/55). {D}rug resistance mutation was found in 41/52 patients in virological failure, for 17.7% (n = 41/231) an overall rate of drug resistance mutations. {M}184{V}/{I} was the most frequent mutation occurring, followed by {K}103{N}. {P}hylogenetic analysis of the 52 genotyped viral isolates showed the predominance of {CRF}02_{AG} with 62% (n = 32/52). {U}se of a {DBS} specimen is suitable to assist national programs for monitoring in remote areas {HIV} drug resistance in resources limited-settings. {J}. {M}ed. {V}irol. 86:45-51, 2014.},
  keywords = {senegal ; dried blood spots ; drug resistance ; {HIV}-1 ; {SENEGAL}},
  booktitle = {},
  journal = {{J}ournal of {M}edical {V}irology},
  volume = {86},
  numero = {1},
  pages = {45--51},
  ISSN = {0146-6615},
  year = {2014},
  DOI = {10.1002/jmv.23778},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061990},
}