@article{fdi:010061975,
  title = {{E}stimating the timing of mother-to-child transmission of the {H}uman {I}mmunodeficiency {V}irus type 1 using a viral molecular evolution model},
  author = {{C}haillon, {A}. and {S}amleerat, {T}. and {Z}oveda, {F}. and {B}allesteros, {S}. and {M}oreau, {A}. and {N}go-{G}iang-{H}uong, {N}icole and {J}ourdain, {G}onzague and {G}ianella, {S}. and {L}allemant, {M}arc and {D}epaulis, {F}. and {B}arin, {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {M}other-to-child transmission ({MTCT}) is responsible for most pediatric {HIV}-1 infections worldwide. {I}t can occur during pregnancy, labor, or breastfeeding. {N}umerous studies have used coalescent and molecular clock methods to understand the epidemic history of {HIV}-1, but the timing of vertical transmission has not been studied using these methods. {T}aking advantage of the constant accumulation of {HIV} genetic variation over time and using longitudinally sampled viral sequences, we used a coalescent approach to investigate the timing of {MTCT}. {M}aterials and {M}ethods: {S}ix-hundred and twenty-two clonal env sequences from the {RNA} and {DNA} viral population were longitudinally sampled from nine {HIV}-1 infected mother-and-child pairs [range: 277-1034 days]. {F}or each transmission pair, timing of {MTCT} was determined using a coalescent-based model within a {B}ayesian statistical framework. {R}esults were compared with available estimates of {MTCT} timing obtained with the classic biomedical approach based on serial {HIV} {DNA} detection by {PCR} assays. {R}esults: {F}our children were infected during pregnancy, whereas the remaining five children were infected at time of delivery. {F}or eight out of nine pairs, results were consistent with the transmission periods assessed by standard {PCR}-based assay. {T}he discordance in the remaining case was likely confused by co-infection, with simultaneous introduction of multiple maternal viral variants at the time of delivery. {C}onclusions: {T}he study provided the opportunity to validate the {B}ayesian coalescent approach that determines the timing of {MTCT} of {HIV}-1. {I}t illustrates the power of population genetics approaches to reliably estimate the timing of transmission events and deepens our knowledge about the dynamics of viral evolution in {HIV}-infected children, accounting for the complexity of multiple transmission events.},
  keywords = {{THAILANDE}},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {9},
  numero = {4},
  pages = {e90421},
  ISSN = {1932-6203},
  year = {2014},
  DOI = {10.1371/journal.pone.0090421},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061975},
}