@article{fdi:010061970,
  title = {{P}opulation genetics of {T}rypanosoma brucei circulating in {G}lossina palpalis palpalis and domestic animals of the {F}ontem sleeping sickness focus of {C}ameroon},
  author = {{S}imo, {G}. and {N}jitchouang, {G}. {R}. and {M}elachio, {T}. {T}. {T}. and {N}jiokou, {F}. and {C}uny, {G}{\'e}rard and {T}azoacha, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {H}uman {A}frican {T}rypanosomiasis is still a public health threat in {C}ameroon. {T}o assess {T}rypanosoma brucei strains circulating in the {F}ontem sleeping sickness focus, we conducted a genetic structure study using microsatellites to assess genotypes circulating in both tsetse flies and domestic animals. {M}ethod: {F}or this study, pyramidal traps were set up and 2695 tsetse flies were collected and 1535 (57%) living flies were dissected and their mid- guts collected. {F}urthermore, blood samples were collected from 397 domestic animals (pigs, goats, sheep and dogs). {DNA} was extracted from midguts and blood samples, and specific primers were used to identify trypanosomes of the subgenus {T}rypanozoon. {A}ll positive samples were genetically characterized with seven microsatellite markers. {R}esults: {S}eventy five (4.7%) midguts of tsetse flies and 140 (35.2%) domestic animals were found infected by trypanosomes of the subgenus {T}rypanozoon. {T}he genetic characterization of 215 {T}rypanozoon positive samples (75 from tsetse and 140 from animals) revealed a genetic diversity between {T}rypanosoma brucei circulating in tsetse and domestic animals. {O}f these positive samples, 87 (40.5%) single infections were used here to investigate the population genetics of {T}rypanosoma brucei circulating in tsetse and domestic animals. {T}he dendrogram illustrating the genetic similarities between {T}rypanosoma brucei genotypes was subdivided into four clusters. {T}he samples from tsetse belonged to the same cluster whereas the samples from domestic animals and espcially pigs were distributed in the four clusters. {C}onclusion: {P}igs appeared as the animal species harboring the highest number of different {T}rypanosoma brucei strains. {T}hey may play an important role in the propagation of different genotypes. {T}he {FST} values revealed a sub structuration of {T}rypanosoma brucei according to hosts and sometimes villages. {T}he data obtained from this study may have considerable importance for the understanding of the transmission and the spread of specific genotypes of {T}rypanosoma brucei.},
  keywords = {{T}rypanosoma brucei ; {T}setse flies ; {D}omestic animals ; {M}icrosatellite markers ; {P}opulation genetics ; {CAMEROUN}},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {7},
  numero = {},
  pages = {art. 156},
  ISSN = {1756-3305},
  year = {2014},
  DOI = {10.1186/1756-3305-7-156},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061970},
}