@article{fdi:010061945,
  title = {{T}he metal transporter {P}g{IREG}1 from the hyperaccumulator {P}sychotria gabriellae is a candidate gene for nickel tolerance and accumulation},
  author = {{M}erlot, {S}. and {H}annibal, {L}aure and {M}artins, {S}. and {M}artinelli, {L}. and {A}mir, {H}. and {L}ebrun, {M}ichel and {T}homine, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{W}e used {RNA}-{S}eq to sequence the transcriptome of the nickel hyperaccumulator {P}sychotria gabriellae endemic from {N}ew {C}aledonia. {U}sing these sequences, we identified the {P}g{IREG}1 transporter as a candidate gene for nickel hyperaccumulation.{N}ickel is an economically important metal and phytotechnologies are being developed to limit the impact of nickel mining on the environment. {M}ore than 300 plant species are known to hyperaccumulate nickel. {H}owever, our knowledge of the mechanisms involved in nickel accumulation in plants is very limited because it has not yet been possible to study these hyperaccumulators at the genomic level. {H}ere, we used next-generation sequencing technologies to sequence the transcriptome of the nickel hyperaccumulator {P}sychotria gabriellae of the {R}ubiaceae family, and used yeast and {A}rabidopsis as heterologous systems to study the activity of identified metal transporters. {W}e characterized the activity of three metal transporters from the {NRAMP} and {IREG}/{FPN} families. {I}n particular, we showed that {P}g{IREG}1 is able to confer nickel tolerance when expressed in yeast and in transgenic plants, where it localizes in the tonoplast. {I}n addition, {P}g{IREG}1 shows higher expression in {P}. gabriellae than in the related non-accumulator species {P}sychotria semperflorens. {O}ur results designate {P}g{IREG}1 as a candidate gene for nickel tolerance and hyperaccumulation in {P}. gabriellae. {T}hese results also show how next-generation sequencing technologies can be used to access the transcriptome of non-model nickel hyperaccumulators to identify the underlying molecular mechanisms.},
  keywords = {{D}e novo sequencing ; {F}erroportin ; hyperaccumulator ; nickel ; {NRAMP} ; {P}sychotria gabriellae ; {NOUVELLE} {CALEDONIE}},
  booktitle = {},
  journal = {{J}ournal of {E}xperimental {B}otany},
  volume = {65},
  numero = {6},
  pages = {1551--1564},
  ISSN = {0022-0957},
  year = {2014},
  DOI = {10.1093/jxb/eru025},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061945},
}