@article{fdi:010061944,
  title = {{E}ffect of activated charcoal on multiplication of {A}frican yam ({D}ioscorea cayenensis-rotundata) nodal segments using a temporary immersion bioreactor ({RITA} ({R}))},
  author = {{P}olzin, {F}. and {S}ylvestre, {I}sabelle and {D}echamp, {E}. and {I}lbert, {P}. and {E}tienne, {H}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n this study, we compared the growth of {D}ioscorea cayenensis-rotundata ({A}frican yam) nodal segments, using semisolid medium in test tubes and liquid medium in 1-{L} {R}ecipient for {A}utomated {T}emporary {I}mmersion ({RITA}(({R}))) temporary immersion bioreactors ({TIB}), and the application of various culture parameters. {T}he addition of activated charcoal ({AC}) had a positive effect on the growth of nodal segments, both in semisolid medium and in liquid medium in {RITA}(({R})) bioreactors. {A}fter 2 mo culture in the presence of {AC}, plantlets were 6.4-6.6 cm long compared to 3.2-3.8 cm in absence of {AC}, with no significant difference observed between the culture systems. {I}n the range of inoculation densities tested (5-20 nodal segments per {RITA}(({R})) bioreactor), there was no effect on the number of buds produced per nodal segment, the moisture content of plantlets (fresh weight basis), or on net fresh weight gain. {B}y contrast, the individual leaf surface area of plantlets decreased in line with increasing inoculation density. {A}mong the range of benzylaminopurine ({BAP}) concentrations tested (0-17.6 mu {M}), 0.44 mu {M} induced the highest number of buds (3.8 buds per nodal segment) in the {TIB}. {H}owever, comparable numbers of buds could be produced with media devoid of {BAP}, either by increasing the frequency of 1-min daily immersion cycles in {RITA}(({R})) bioreactors from one every 12 h to one every 4 h or by using semisolid medium containing {AC}.},
  keywords = {{A}ctivated charcoal ; {B}enzylaminopurine ; {D}ioscorea cayenensis-rotundata ; {N}odal segments ; {T}emporary immersion ; {RITA} ({R}) bioreactors},
  booktitle = {},
  journal = {{I}n {V}itro {C}ellular and {D}evelopmental {B}iology-{P}lant},
  volume = {50},
  numero = {2},
  pages = {210--216},
  ISSN = {1054-5476},
  year = {2014},
  DOI = {10.1007/s11627-013-9552-6},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061944},
}