@article{fdi:010061928,
  title = {{DNA} methylation and expression of the {E}g{DEF}1 gene and neighboring retrotransposons in mantled somaclonal variants of oil palm},
  author = {{J}aligot, {E}. and {H}ooi, {W}. {Y}. and {D}ebladis, {E}. and {R}ichaud, {F}. and {B}eule, {T}. and {C}ollin, {M}yriam and {A}gbessi, {M}. {D}. {T}. and {S}abot, {F}ran{\c{c}}ois and {G}arsmeur, {O}. and {D}'{H}ont, {A}. and {A}lwee, {S}. {S}. {R}. {S}. and {R}ival, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he mantled floral phenotype of oil palm ({E}laeis guineensis) affects somatic embryogenesis-derived individuals and is morphologically similar to mutants defective in the {B}-class {MADS}-box genes. {T}his somaclonal variation has been previously demonstrated to be associated to a significant deficit in genome-wide {DNA} methylation. {I}n order to elucidate the possible role of {DNA} methylation in the transcriptional regulation of {E}g{DEF}1, the {APETALA}3 ortholog of oil palm, we studied this epigenetic mark within the gene in parallel with transcript accumulation in both normal and mantled developing inflorescences. {W}e also examined the methylation and expression of two neighboring retrotransposons that might interfere with {E}g{DEF}1 regulation. {W}e show that the {E}g{DEF}1 gene is essentially unmethylated and that its methylation pattern does not change with the floral phenotype whereas expression is dramatically different, ruling out a direct implication of {DNA} methylation in the regulation of this gene. {A}lso, we find that both the gypsy element inserted within an intron of the {E}g{DEF}1 gene and the copia element located upstream from the promoter are heavily methylated and show little or no expression. {I}nterestingly, we identify a shorter, alternative transcript produced by {E}g{DEF}1 and characterize its accumulation with respect to its full-length counterpart. {W}e demonstrate that, depending on the floral phenotype, the respective proportions of these two transcripts change differently during inflorescence development. {W}e discuss the possible phenotypical consequences of this alternative splicing and the new questions it raises in the search for the molecular mechanisms underlying the mantled phenotype in the oil palm.},
  keywords = {{MALAISIE} ; {COTE} {D}'{IVOIRE}},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {9},
  numero = {3},
  pages = {e91896 [14 ]},
  ISSN = {1932-6203},
  year = {2014},
  DOI = {10.1371/journal.pone.0091896},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061928},
}