@article{fdi:010061912,
  title = {{H}uman {I}g{G} antibody response to {A}edes aegypti {N}term-34 k{D}a salivary peptide as an indicator to identify areas at high risk for dengue transmission : a retrospective study in urban settings of {V}ientiane city, {L}ao {PDR}},
  author = {{N}dille, {E}. {E}. and {D}ubot {P}{\'e}r{\`e}s, {A}udrey and {D}oucour{\'e}, {S}ouleymane and {M}ouchet, {F}ran{\c{c}}ois and {C}orn{\'e}lie, {S}ylvie and {S}idavong, {B}. and {F}ournet, {F}lorence and {R}emou{\'e}, {F}ranck},
  editor = {},
  language = {{ENG}},
  abstract = {{O}bjective{U}sing human {I}g{G} antibody response to the {A}edes {N}term-34k{D}a salivary peptide as an indicator of human exposure to {A}edes bites in surveying exposed populations from areas at risk of dengue virus ({DENV}) transmission in urban settings of {V}ientiane city, {L}ao {PDR}. {M}ethods{E}nzyme-linked immunosorbent assay tests were performed to measure the {I}g{G} response to {N}term-34k{D}a peptide in blood samples collected within a flavivirus seroprevalence survey carried out in 2006 including 3558 randomly selected individuals. {T}he level of {I}g{G} response to the {N}term-34k{D}a peptide in individuals was analysed in relation to the level of urbanisation of the individual's residence, areas that presented significant differences in the prevalence of recent {DENV} infection. {R}esults{N}o differences were observed in the anti-{N}term-34k{D}a {I}g{G} level between {DENV}-positive and {DENV}-negative individuals. {H}owever, the level of specific {I}g{G} response was higher among individuals living in slightly urbanised neighbourhoods than among those in more highly urbanised areas ({P}<0.0001). {I}nterestingly, a similar pattern had already been observed concerning the prevalence of recent {DENV} infection in the same populations. {C}onclusion{T}he results of this retrospective study indicate that the evaluation of human {I}g{G} response to the {A}edes {N}term-34k{D}a salivary peptide could be a useful indicator to identify places with risk of dengue virus transmission in urban endemic areas. {O}bjectif{U}tiliser la reponse de l'anticorps {I}g{G} humaine au peptide salivaire {N}term-34k{D}a de l'{A}edes comme un indicateur de l'exposition humaine aux piqures de l'{A}edes dans la surveillance des populations exposees dans les zones a risque de transmission du virus de la dengue ({DENV}) en milieu urbain de la ville de {V}ientiane, au {L}aos. {M}ethodes{D}es tests {ELISA} ont ete effectues pour mesurer la reponse {I}g{G} au peptide {N}term-34k{D}a dans des echantillons de sang preleves dans une etude de seroprevalence du flavivirus realisee en 2006, sur 3558 personnes choisies aleatoirement. {L}e niveau de reponse {I}g{G} contre le peptide {N}term-34k{D}a chez les individus a ete analyse en relation avec le niveau d'urbanisation du lieu de residence des individus, les zones qui presentaient des differences significatives dans la prevalence de l'infection {DENV} recente. {R}esultats{A}ucune difference n'a ete observee dans le niveau d'{I}g{G} anti-{N}term-34k{D}a entre les individus positifs et negatifs pour le {DENV}. {C}ependant, le niveau de la reponse {I}g{G} specifique etait plus eleve chez les personnes vivant dans des quartiers un peu urbanises que chez ceux vivant dans des zones plus fortement urbanisees ({P}<0,0001). {D}e facon interessante, une tendance similaire avait deja ete observee dans la prevalence de l'infection {DENV} recente dans les memes populations. {C}onclusion{L}es resultats de cette etude retrospective indiquent que l'evaluation de la reponse {I}g{G} humaine au peptide salivaire {N}term-34k{D}a d'{A}edes pourrait etre un indicateur utile pour identifier les zones a risque de transmission du virus de la dengue dans les zones endemiques urbaines. {O}bjetivo{U}tilizando la respuesta de anticuerpos {I}g{G} humanos al peptido salivar {N}term-34k{D}a de {A}edes como un indicador de la exposicion humana a picaduras de {A}edes, estudiar las poblaciones expuestas pertenecientes a areas en riesgo de transmision del virus del dengue ({VDEN}) en zonas urbanas de la ciudad de {V}ientiane, {L}aos. {M}etodos{S}e realizaron pruebas de {ELISA} para medir la respuesta de {I}g{G} al peptido {N}term-34k{D}a en muestras de sangre recogidas como parte de un estudio de seroprevalencia de flavivirus llevado a cabo en el 2006, incluyendose 3558 individuos escogidos al azar. {S}e analizo el nivel de respuesta de {I}g{G} al peptido {N}term-34k{D}a con relacion al nivel de urbanizacion de la residencia del individuo, areas que presentaron diferencias significativas en la prevalencia de infecciones recientes por {VDEN}. {R}esultados{N}o se observaron diferencias entre el nivel de {I}g{G} anti-{N}term-34k{D}a entre individuos positivos y negativos para {VDEN}. {S}in embargo, el nivel de respuesta {I}g{G} especifica era mayor entre individuos viviendo en barrios relativamente urbanizados, que entre aquellos en areas muy urbanizadas ({P}<0.0001). {S}e observo un patron similar con respecto a la prevalencia de infecciones recientes de {VDEN} entre la misma poblacion. {C}onclusion{L}os resultados de este estudio retrospectivo indican que la evaluacion de la respuesta de {I}g{G} humana al peptido salivar {N}term-34k{D}a de {A}edes podria ser un indicador util para identificar areas con riesgo de transmision del virus del dengue en areas endemicas urbanas.},
  keywords = {salivary biomarker ; {A}edes bites ; dengue risk ; spatial distribution ; residential neighbourhoods ; {V}ientiane ; {LAOS}},
  booktitle = {},
  journal = {{T}ropical {M}edicine and {I}nternational {H}ealth},
  volume = {19},
  numero = {5},
  pages = {576--580},
  ISSN = {1360-2276},
  year = {2014},
  DOI = {10.1111/tmi.12280},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061912},
}