@article{fdi:010061886,
  title = {{SYBR} green real-time {PCR} for the detection of all enterovirus-{A}71 genogroups},
  author = {{D}ubot {P}{\'e}r{\`e}s, {A}udrey and {T}an, {C}. {Y}. {Q}. and de {C}hesse, {R}. and {S}ibounheuang, {B}. and {V}ongsouvath, {M}. and {P}hommasone, {K}. and {B}essaud, {M}. and {G}azin, {C}. and {T}hirion, {L}aurence and {P}hetsouvanh, {R}. and {N}ewton, {P}. {N}. and de {L}amballerie, {X}avier},
  editor = {},
  language = {{ENG}},
  abstract = {{E}nterovirus {A}71 ({EV}-{A}71) has recently become an important public health threat, especially in {S}outh-{E}ast {A}sia, where it has caused massive outbreaks of {H}and, {F}oot and {M}outh disease every year, resulting in significant mortality. {R}apid detection of {EV}-{A}71 early in outbreaks would facilitate implementation of prevention and control measures to limit spread. {R}eal-time {RTPCR} is the technique of choice for the rapid diagnosis of {EV}-{A}71 infection and several systems have been developed to detect circulating strains. {A}lthough eight genogroups have been described globally, none of these {PCR} techniques detect all eight. {W}e describe, for the first time, a {SYBR} {G}reen real-time {RT}-{PCR} system validated to detect all 8 {EV}-{A}71 genogroups. {T}his tool could permit the early detection and shift in genogroup circulation and the standardization of {HFMD} virological diagnosis, facilitating networking of laboratories working on {EV}-{A}71 in different regions.},
  keywords = {{LAOS}},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {9},
  numero = {3},
  pages = {e89963},
  ISSN = {1932-6203},
  year = {2014},
  DOI = {10.1371/journal.pone.0089963},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061886},
}