Publications des scientifiques de l'IRD

Dubot Pérès Audrey, Tan C. Y. Q., de Chesse R., Sibounheuang B., Vongsouvath M., Phommasone K., Bessaud M., Gazin C., Thirion Laurence, Phetsouvanh R., Newton P. N., de Lamballerie Xavier. (2014). SYBR green real-time PCR for the detection of all enterovirus-A71 genogroups. Plos One, 9 (3), p. e89963. ISSN 1932-6203.

Titre du document
SYBR green real-time PCR for the detection of all enterovirus-A71 genogroups
Année de publication
2014
Type de document
Article référencé dans le Web of Science WOS:000333352800006
Auteurs
Dubot Pérès Audrey, Tan C. Y. Q., de Chesse R., Sibounheuang B., Vongsouvath M., Phommasone K., Bessaud M., Gazin C., Thirion Laurence, Phetsouvanh R., Newton P. N., de Lamballerie Xavier
Source
Plos One, 2014, 9 (3), p. e89963 ISSN 1932-6203
Enterovirus A71 (EV-A71) has recently become an important public health threat, especially in South-East Asia, where it has caused massive outbreaks of Hand, Foot and Mouth disease every year, resulting in significant mortality. Rapid detection of EV-A71 early in outbreaks would facilitate implementation of prevention and control measures to limit spread. Real-time RTPCR is the technique of choice for the rapid diagnosis of EV-A71 infection and several systems have been developed to detect circulating strains. Although eight genogroups have been described globally, none of these PCR techniques detect all eight. We describe, for the first time, a SYBR Green real-time RT-PCR system validated to detect all 8 EV-A71 genogroups. This tool could permit the early detection and shift in genogroup circulation and the standardization of HFMD virological diagnosis, facilitating networking of laboratories working on EV-A71 in different regions.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020] ; Santé : généralités [050] ; Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
LAOS
Localisation
Fonds IRD [F B010061886]
Identifiant IRD
fdi:010061886
Contact