@article{fdi:010061849,
  title = {{P}olyphasic analysis of a middle ages coprolite microbiota, {B}elgium},
  author = {{A}ppelt, {S}. and {A}rmougom, {F}abrice and {L}e {B}ailly, {M}. and {R}obert, {C}. and {D}rancourt, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{P}aleomicrobiological investigations of a 14th-century coprolite found inside a barrel in {N}amur, {B}elgium were done using microscopy, a culture-dependent approach and metagenomics. {R}esults were confirmed by ad hoc {PCR} - sequencing. {I}nvestigations yielded evidence for flora from ancient environment preserved inside the coprolite, indicated by microscopic observation of amoebal cysts, plant fibers, seeds, pollens and mold remains. {S}eventeen different bacterial species were cultured from the coprolite, mixing organisms known to originate from the environment and organisms known to be gut inhabitants. {M}etagenomic analyses yielded 107,470 reads, of which known sequences (31.9%) comprised 98.98% bacterial, 0.52% eukaryotic, 0.44% archaeal and 0.06% viral assigned reads. {M}ost abundant bacterial phyla were {P}roteobacteria, {G}emmatimonadetes, {A}ctinobacteria and {B}acteroidetes. {T}he 16 {S} r{RNA} gene dataset yielded 132,000 trimmed reads and 673 {O}perational {T}axonomic {U}nits. {M}ost abundant bacterial phyla observed in the 16 {S} r{RNA} gene dataset belonged to {P}roteobacteria, {F}irmicutes, {A}ctinobacteria and {C}hlamydia. {T}he {N}amur coprolite yielded typical gut microbiota inhabitants, intestinal parasites {T}richuris and {A}scaris and systemic pathogens {B}artonella and {B}ordetella. {T}his study adds knowledge to gut microbiota in medieval times.},
  keywords = {{BELGIQUE}},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {9},
  numero = {2},
  pages = {art. e88376 [8 ]},
  ISSN = {1932-6203},
  year = {2014},
  DOI = {10.1371/journal.pone.0088376},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061849},
}