@article{fdi:010061832,
  title = {{T}olerance of apexes of coral {P}ocillopora damicornis {L}. to cryoprotectant solutions},
  author = {{F}euillassier, {L}. and {R}omans, {P}. and {S}ylvestre, {I}sabelle and {M}asanet, {P}. and {B}arthelemy, {D}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n this study, we investigated the tolerance of {P}ocillopora damicornis apexes to treatments with solutions containing penetrating and non-penetrating cryoprotective agents ({CPA}s). {CPA}s were employed individually or in binary, tertiary or quaternary solutions. {I}n some experiments apexes were treated successively with two {CPA} solutions with increasing total concentration. {P}. damicornis apexes withstood exposure for up to 30 min to solutions containing 0.6-0.8 {M} sucrose ({S}uc) or trehalose ({T}re). {W}hen apexes were treated with binary cryoprotectant solutions containing {S}uc and ethylene glycol ({EG}), methanol ({M}eth), dimethyl sulfoxide ({M}e2{SO}) or glycerol ({G}ly), the {CPA}s employed in combination with {S}uc could be ranked in the following order of decreasing tolerance: {EG} > {M}eth > {M}e2{SO} > {G}ly. {P}. damicornis apexes tolerated exposure to complex {CPA} solutions containing {S}uc, {M}e2{SO}, {EG} and/or {M}eth with a total molarity of 2.45 {M}. {I}n experiments where two successive {CPA} solutions were employed, apexes withstood treatment with the second, more concentrated solution at 0 degrees {C} for up to 10 min. {T}hese preliminary results pave the way to the development of a cryopreservation protocol for {P}. damicornis apexes.},
  keywords = {{C}oral ; {P}ocillopora damicornis ; {C}ryopreservation ; {A}pex ; {C}ryoprotectant ; solutions},
  booktitle = {},
  journal = {{C}ryobiology},
  volume = {68},
  numero = {1},
  pages = {96--106},
  ISSN = {0011-2240},
  year = {2014},
  DOI = {10.1016/j.cryobiol.2014.01.001},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061832},
}