@article{fdi:010061831,
  title = {{H}-2-dependent azoreduction by {S}hewanella oneidensis {MR}-1 : involvement of secreted flavins and both [{N}i-{F}e] and [{F}e-{F}e] hydrogenases},
  author = {{L}e {L}az, {S}. and {K}pebe, {A}. and {L}orquin, {J}ean and {B}rugna, {M}. and {R}ousset, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n this paper, the hydrogen ({H}-2)-dependent discoloration of azo dye amaranth by {S}hewanella oneidensis {MR}-1 was investigated. {E}xperiments with hydrogenase-deficient strains demonstrated that periplasmic [{N}i-{F}e] hydrogenase ({H}ya{B}) and periplasmic [{F}e-{F}e] hydrogenase ({H}yd{A}) are both respiratory hydrogenases of dissimilatory azoreduction in {S}. oneidensis {MR}-1. {T}hese findings suggest that {H}ya{B} and {H}yd{A} can function as uptake hydrogenases that couple the oxidation of {H}-2 to the reduction of amaranth to sustain cellular growth. {T}his constitutes to our knowledge the first report of the involvement of [{F}e-{F}e] hydrogenase in a bacterial azoreduction process. {A}ssays with respiratory inhibitors indicated that a menaquinone pool and different cytochromes were involved in the azoreduction process. {H}igh-performance liquid chromatography analysis revealed that flavin mononucleotide and riboflavin were secreted in culture supernatant by {S}. oneidensis {MR}-1 under {H}-2-dependent conditions with concentration of 1.4 and 2.4 mu mol g protein(-1), respectively. {T}hese endogenous flavins were shown to significantly accelerate the reduction of amaranth at micromolar concentrations acting as electron shuttles between the cell surface and the extracellular azo dye. {T}his work may facilitate a better understanding of the mechanisms of azoreduction by {S}. oneidensis {MR}-1 and may have practical applications for microbiological treatments of dye-polluted industrial effluents.},
  keywords = {{S}hewanella oneidensis {MR}-1 ; {A}maranth ; {H}ydrogen ; {A}zo dye ; {F}lavins},
  booktitle = {},
  journal = {{A}pplied {M}icrobiology and {B}iotechnology},
  volume = {98},
  numero = {6},
  pages = {2699--2707},
  ISSN = {0175-7598},
  year = {2014},
  DOI = {10.1007/s00253-013-5208-z},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061831},
}