@article{fdi:010061830,
  title = {{B}reaking dogmas : the plant vascular pathogen {X}anthomonas albilineans is able to invade non-vascular tissues despite its reduced genome},
  author = {{M}ensi, {I}. and {V}ernerey, {M}. {S}. and {G}argani, {D}. and {N}icole, {M}ichel and {R}ott, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{X}anthomonas albilineans, the causal agent of sugarcane leaf scald, is missing the {H}rp type {III} secretion system that is used by many {G}ram-negative bacteria to colonize their host. {U}ntil now, this pathogen was considered as strictly limited to the xylem of sugarcane. {W}e used confocal laser scanning microscopy, immunocytochemistry and transmission electron microscopy ({TEM}) to investigate the localization of {X}. albilineans in diseased sugarcane. {S}ugarcane plants were inoculated with strains of the pathogen labelled with a green fluorescent protein. {C}onfocal microscopy observations of symptomatic leaves confirmed the presence of the pathogen in the protoxylem and metaxylem; however, {X}. albilineans was also observed in phloem, parenchyma and bulliform cells of the infected leaves. {S}imilarly, vascular bundles of infected sugarcane stalks were invaded by {X}. albilineans. {S}urprisingly, the pathogen was also observed in apparently intact storage cells of the stalk and in intercellular spaces between these cells. {M}ost of these observations made by confocal microscopy were confirmed by {TEM}. {T}he pathogen exits the xylem following cell wall and middle lamellae degradation, thus creating openings to reach parenchyma cells. {T}his is the first description of a plant pathogenic vascular bacterium invading apparently intact non-vascular plant tissues and multiplying in parenchyma cells.},
  keywords = {{X}anthomonas albilineans ; confocal laser scanning microscopy ; cytochemistry ; transmission electron microscopy ; non-vascular plant tissue},
  booktitle = {},
  journal = {{O}pen {B}iology},
  volume = {4},
  numero = {},
  pages = {art. 130116 [13 ]},
  ISSN = {2046-2441},
  year = {2014},
  DOI = {10.1098/rsob.130116},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061830},
}