@article{fdi:010061811,
  title = {{E}xpression profiles of key phenylpropanoid genes during {V}anilla planifolia pod development reveal a positive correlation between {PAL} gene expression and vanillin biosynthesis},
  author = {{F}ock-{B}astide, {I}. and {P}alama, {T}. {L}. and {B}ory, {S}. and {L}ecolier, {A}. and {N}oirot, {M}ichel and {J}o{\¨e}t, {T}hierry},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n {V}anilla planifolia pods, development of flavor precursors is dependent on the phenylpropanoid pathway. {T}he distinctive vanilla aroma is produced by numerous phenolic compounds of which vanillin is the most important. {B}ecause of the economic importance of vanilla, vanillin biosynthetic pathways have been extensively studied but agreement has not yet been reached on the processes leading to its accumulation. {I}n order to explore the transcriptional control exerted on these pathways, five key phenylpropanoid genes expressed during pod development were identified and their m{RNA} accumulation profiles were evaluated during pod development and maturation using quantitative real-time {PCR}. {A}s a prerequisite for expression analysis using q{RT}-{PCR}, five potential reference genes were tested, and two genes encoding {A}ctin and {EF}1 were shown to be the most stable reference genes for accurate normalization during pod development. {F}or the first time, genes encoding a phenylalanine ammonia-lyase ({V}p{PAL}1) and a cinnamate 4-hydroxylase ({V}p{C}4{H}1) were identified in vanilla pods and studied during maturation. {A}mong phenylpropanoid genes, differential regulation was observed from 3 to 8 months after pollination. {V}p{PAL}1 was gradually up-regulated, reaching the maximum expression level at maturity. {I}n contrast, genes encoding 4{HBS}, {C}4{H}, {OMT}2 and {OMT}3 did not show significant increase in expression levels after the fourth month post-pollination. {E}xpression profiling of these key phenylpropanoid genes is also discussed in light of accumulation patterns for key phenolic compounds. {I}nterestingly, {V}p{PAL}1 gene expression was shown to be positively correlated to maturation and vanillin accumulation.},
  keywords = {{C}innamate 4-hydroxylase ; {F}ruit maturation ; {P}henolic compounds ; {P}henylalanine ammonia-lyase ; {R}eal-time quantitative {PCR} ; {R}eference genes ; {V}anilla planifolia ; {REUNION}},
  booktitle = {},
  journal = {{P}lant {P}hysiology and {B}iochemistry},
  volume = {74},
  numero = {},
  pages = {304--314},
  ISSN = {0981-9428},
  year = {2014},
  DOI = {10.1016/j.plaphy.2013.11.026},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061811},
}