Horizon / Plein textes La base de ressources documentaires de l'IRD

IRD

 

Publications des scientifiques de l'IRD

Campbell M. C., Ranciaro A., Zinshteyn D., Rawlings-Goss R., Hirbo J., Thompson S., Woldemeskel D., Froment Alain, Rucker J. B., Omar S. A., Bodo J. M., Nyambo T., Belay G., Drayna D., Breslin P. A. S., Tishkoff S. A. (2014). Origin and differential selection of allelic variation at TAS2R16 associated with salicin bitter taste sensitivity in Africa. Molecular Biology and Evolution, 31 (2), 288-302. ISSN 0737-4038

Accès réservé (Intranet IRD) Demander le PDF

Lien direct chez l'éditeur doi:10.1093/molbev/mst211

Titre
Origin and differential selection of allelic variation at TAS2R16 associated with salicin bitter taste sensitivity in Africa
Année de publication2014
Type de documentArticle référencé dans le Web of Science WOS:000330836900004
AuteursCampbell M. C., Ranciaro A., Zinshteyn D., Rawlings-Goss R., Hirbo J., Thompson S., Woldemeskel D., Froment Alain, Rucker J. B., Omar S. A., Bodo J. M., Nyambo T., Belay G., Drayna D., Breslin P. A. S., Tishkoff S. A.
SourceMolecular Biology and Evolution, 2014, 31 (2), p. 288-302. ISSN 0737-4038
RésuméBitter taste perception influences human nutrition and health, and the genetic variation underlying this trait may play a role in disease susceptibility. To better understand the genetic architecture and patterns of phenotypic variability of bitter taste perception, we sequenced a 996 bp region, encompassing the coding exon of TAS2R16, a bitter taste receptor gene, in 595 individuals from 74 African populations and in 94 non-Africans from 11 populations. We also performed genotype-phenotype association analyses of threshold levels of sensitivity to salicin, a bitter anti-inflammatory compound, in 296 individuals from Central and East Africa. In addition, we characterized TAS2R16 mutants in vitro to investigate the effects of polymorphic loci identified at this locus on receptor function. Here, we report striking signatures of positive selection, including significant Fay and Wu's H statistics predominantly in East Africa, indicating strong local adaptation and greater genetic structure among African populations than expected under neutrality. Furthermore, we observed a "star-like" phylogeny for haplotypes with the derived allele at polymorphic site 516 associated with increased bitter taste perception that is consistent with a model of selection for "high-sensitivity" variation. In contrast, haplotypes carrying the "low-sensitivity" ancestral allele at site 516 showed evidence of strong purifying selection. We also demonstrated, for the first time, the functional effect of nonsynonymous variation at site 516 on salicin phenotypic variance in vivo in diverse Africans and showed that most other nonsynonymous substitutions have weak or no effect on cell surface expression in vitro, suggesting that one main polymorphism at TAS2R16 influences salicin recognition. Additionally, we detected geographic differences in levels of bitter taste perception in Africa not previously reported and infer an East African origin for high salicin sensitivity in human populations.
Plan de classementNutrition, alimentation [054] ; Société, développement social [106] ; Sciences fondamentales / Techniques d'analyse et de recherche [020]
Descr. géo.AFRIQUE
LocalisationFonds IRD [F B010061791]
Identifiant IRDfdi:010061791
Lien permanenthttp://www.documentation.ird.fr/hor/fdi:010061791

Export des données

Disponibilité des documents

Télechargment fichier PDF téléchargeable

Lien sur le Web lien chez l'éditeur

Accès réservé en accès réservé

HAL en libre accès sur HAL


Accès aux documents originaux :

Le FDI est labellisé CollEx

Accès direct

Bureau du chercheur

Site de la documentation

Espace intranet IST (accès réservé)

Suivi des publications IRD (accès réservé)

Mentions légales

Services Horizon

Poser une question

Consulter l'aide en ligne

Déposer une publication (accès réservé)

S'abonner au flux RSS

Voir les tableaux chronologiques et thématiques

Centres de documentation

Bondy

Montpellier (centre IRD)

Montpellier (MSE)

Cayenne

Nouméa

Papeete

Abidjan

Dakar

Niamey

Ouagadougou

Tunis

La Paz

Quito