Romero G., Vasquez L. M., Lashermes Philippe, Herrera J. C. (2014). Identification of a major QTL for adult plant resistance to coffee leaf rust (Hemileia vastatrix) in the natural Timor hybrid (Coffea arabica x C. canephora). Plant Breeding, 133 (1), p. 121-129. ISSN 0179-9541.
Titre du document
Identification of a major QTL for adult plant resistance to coffee leaf rust (Hemileia vastatrix) in the natural Timor hybrid (Coffea arabica x C. canephora)
Romero G., Vasquez L. M., Lashermes Philippe, Herrera J. C.
Source
Plant Breeding, 2014,
133 (1), p. 121-129 ISSN 0179-9541
Most of the commercial varieties of coffee (Coffeaarabica L.) derived from the Timor hybrid (TH) have been shown to contain major genes for coffee leaf rust (CLR) resistance. To identify markers tightly linked to such genes, an F-2 mapping population derived from a cross between Caturra' (susceptible variety) and the TH-derived DI.200 line (highly resistant) was generated. Using expressed sequence information and a bioinformatics approach, both targeted region amplified polymorphism (TRAPs) markers and simple sequence repeat (SSR) markers were identified. Phenotypic evaluations in the field and under controlled conditions confirmed the existence of one quantitative trait locus for CLR resistance. Four candidate SSR markers were associated with high CLR resistance. They spanning a region of 2.5cM designated Q(CLR_4) located within chromosome 4 of the international C.canephora map. The presence of this region was confirmed in a set of elite lines and commercial varieties. The Q(CLR_4) region corresponds to a new and genetically independent S-H locus that could potentially be useful in gene pyramiding with other genes to enhance rust resistance in TH derivatives.
