@article{fdi:010061603,
  title = {{A} new method to measure allyl isothiocyanate ({AITC}) concentrations in mustard : comparison of {AITC} and commercial mustard solutions as earthworm extractants},
  author = {{P}elosi, {C}. and {C}hiron, {F}. and {D}ubs, {F}lorence and {H}edde, {M}. and {P}onge, {J}.{F}. and {S}almon, {S}. and {C}luzeau, {D}. and {N}{\'e}lieu, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{E}arthworms are target organisms both for scientists studying the biological component of soils and for farmers concerned with monitoring the quality of their soils. {D}ifferent expellants are used to extract earthworms from the soil but differences in chemical properties and efficiency between commercial mustard and allyl isothiocyanate ({AITC}) solutions remain unknown. {T}he objectives of this study were to compare (i) the concentration of irritating product (allyl isothiocyanate {AITC}) in two expellant solutions (diluted mustard or {AITC} solution) and (ii) their efficiency in extracting earthworms from the soil. {AITC} concentration was analyzed according to a new method, based on {AITC} solvent extraction and {HPLC} quantification, in one commercial mustard brand to assess its variability within and between batches of jars. {A}ccording to mustard spiking with {AITC} standard solution, extraction recovery was estimated as 98 ± 2%. {E}arthworm field data were collected in spring 2012 in 22 cultivated fields located in east Île-de-{F}rance, comparing pure {AITC} to commercial mustard solutions. {S}pecies diversity, abundance and biomass of earthworms per plot were measured. {W}e showed that {AITC} concentration in commercial mustard varied according to the use by date but not according to the batch. {W}e thus recommend using the freshest mustard available from the same batch. {M}oreover, {AITC} solution was found to be about four times more concentrated in {AITC} than the commercial mustard solution. {D}espite this result, no significant differences were found in the efficiency of commercial mustard or {AITC} solutions to bring earthworms to the soil surface in terms of abundance, biomass or diversity. {W}e thus discuss the advantage and drawbacks of using both expellants in the field.},
  keywords = {{LOMBRIC} ; {EXTRACTION} ; {EFFICACITE} ; {CHROMATOGRAPHIE} {EN} {PHASE} {LIQUIDE} ; {BIOMASSE} ; {ABONDANCE} ; {DIVERSITE} {SPECIFIQUE} ; {ETUDE} {EXPERIMENTALE} ; {ETUDE} {COMPARATIVE} ; {MOUTARDE} ; {ALLYL} {ISOTHIOCYANATE} ; {FRANCE} ; {ILE} {DE} {FRANCE} ; {SEINE} {ET} {MARNE}},
  booktitle = {},
  journal = {{A}pplied {S}oil {E}cology},
  volume = {80},
  numero = {},
  pages = {1--5},
  ISSN = {0929-1393},
  year = {2014},
  DOI = {10.1016/j.apsoil.2014.03.005},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061603},
}