@article{fdi:010061571,
  title = {{R}adiation of the {N}od-independent {A}eschynomene relies on multiple allopolyploid speciation events},
  author = {{A}rrighi, {J}ean-{F}ran{\c{c}}ois and {C}haintreuil, {C}l{\'e}mence and {C}artieaux, {F}abienne and {C}ardi, {C}. and {R}odier-{G}oud, {M}. and {B}rown, {S}.{C}. and {B}oursot, {M}arc and {D}'{H}ont, {A}. and {D}reyfus, {B}ernard and {G}iraud, {E}ric},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he semi-aquatic legumes belonging to the genus {A}eschynomene constitute a premium system for investigating the origin and evolution of unusual symbiotic features such as stem nodulation and the presence of a {N}od-independent infection process. {T}his latter apparently arose in a single {A}eschynomene lineage. {B}ut how this unique {N}od-independent group then radiated is not yet known. {W}e have investigated the role of polyploidy in {A}eschynomene speciation via a case study of the pantropical {A}.indica and then extended the analysis to the other {N}od-independent species. {F}or this, we combined {SSR} genotyping, genome characterization through flow cytometry, chromosome counting, {FISH} and {GISH} experiments, molecular phylogenies using {ITS} and single nuclear gene sequences, and artificial hybridizations. {T}hese analyses demonstrate the existence of an {A}.indica polyploid species complex comprising {A}.evenia ({C}. {W}right) (2n=2x=20), {A}.indica {L}. s.s. (2n=4x=40) and a new hexaploid form (2n=6x=60). {T}his latter contains the two genomes present in the tetraploid ({A}.evenia and {A}.scabra) and another unidentified genome. {T}wo other species, {A}.pratensis and {A}.virginica, are also shown to be of allopolyploid origin. {T}his work reveals multiple hybridization/polyploidization events, thus highlighting a prominent role of allopolyploidy in the radiation of the {N}od-independent {A}eschynomene.},
  keywords = {{LEGUMINEUSE} {TROPICALE} ; {SYMBIOSE} ; {NODULE} {RACINAIRE} ; {GENOTYPE} ; {PHYLOGENIE} ; {POLYPLOIDE} ; {HYBRIDATION} ; {BIOLOGIE} {MOLECULAIRE} ; {FLUORESCENCE} ; {ETUDE} {EXPERIMENTALE} ; {ETUDE} {COMPARATIVE} ; {GENOME} ; {TECHNIQUE} {PCR}},
  booktitle = {},
  journal = {{N}ew {P}hytologist},
  volume = {201},
  numero = {4},
  pages = {1457--1468},
  ISSN = {1469-8137},
  year = {2014},
  DOI = {10.1111/nph.12594},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061571},
}