@article{fdi:010061468,
  title = {{D}evelopment validation of a microarray for the investigation of the {CAZ}ymes encoded by the human gut microbiome},
  author = {{E}l {K}aoutari, {A}. and {A}rmougom, {F}abrice and {L}eroy, {Q}. and {V}ialettes, {B}. and {M}illion, {M}. and {R}aoult, {D}idier and {H}enrissat, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{D}istal gut bacteria play a pivotal role in the digestion of dietary polysaccharides by producing a large number of carbohydrate-active enzymes ({CAZ}ymes) that the host otherwise does not produce. {W}e report here the design of a custom microarray that we used to spot non-redundant {DNA} probes for more than 6,500 genes encoding glycoside hydrolases and lyases selected from 174 reference genomes from distal gut bacteria. {T}he custom microarray was tested and validated by the hybridization of bacterial {DNA} extracted from the stool samples of lean, obese and anorexic individuals. {O}ur results suggest that a microarray-based study can detect genes from low-abundance bacteria better than metagenomic-based studies. {A} striking example was the finding that a gene encoding a {GH}6-family cellulase was present in all subjects examined, whereas metagenomic studies have consistently failed to detect this gene in both human and animal gut microbiomes. {I}n addition, an examination of eight stool samples allowed the identification of a corresponding {CAZ}ome core containing 46 families of glycoside hydrolases and polysaccharide lyases, which suggests the functional stability of the gut microbiota despite large taxonomical variations between individuals.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {8},
  numero = {12},
  pages = {e84033},
  ISSN = {1932-6203},
  year = {2013},
  DOI = {10.1371/journal.pone.0084033},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061468},
}