@article{fdi:010061450,
  title = {{B}iochemical, transcriptional and translational evidences of the phenol-meta-degradation pathway by the hyperthermophilic {S}ulfolobus solfataricus 98/2},
  author = {{C}omte, {A}. and {C}hristen, {P}ierre and {D}avidson, {S}ylvain and {P}ophillat, {M}. and {L}orquin, {J}ean and {A}uria, {R}ichard and {S}imon, {G}. and {C}asalot, {L}aurence},
  editor = {},
  language = {{ENG}},
  abstract = {{P}henol is a widespread pollutant and a model molecule to study the biodegradation of monoaromatic compounds. {A}fter a first oxidation step leading to catechol in mesophilic and thermophilic microorganisms, two main routes have been identified depending on the cleavage of the aromatic ring: ortho involving a catechol 1,2 dioxygenase ({C}12{D}) and meta involving a catechol 2,3 dioxygenase ({C}23{D}). {O}ur work aimed at elucidating the phenol-degradation pathway in the hyperthermophilic archaea {S}ulfolobus solfataricus 98/2. {F}or this purpose, the strain was cultivated in a fermentor under different substrate and oxygenation conditions. {I}ndeed, reducing dissolved-oxygen concentration allowed slowing down phenol catabolism (specific growth and phenol-consumption rates dropped 55% and 39%, respectively) and thus, evidencing intermediate accumulations in the broth. {HPLC}/{D}iode {A}rray {D}etector and {LC}-{MS} analyses on culture samples at low dissolved-oxygen concentration ({DOC} = 0.06 mg.{L}-1) suggested, apart for catechol, the presence of 2-hydroxymuconic acid, 4-oxalocrotonate and 4-hydroxy-2-oxovalerate, three intermediates of the meta route. {RT}-{PCR} analysis on oxygenase-coding genes of {S}. solfataricus 98/2 showed that the gene coding for the {C}23{D} was expressed only on phenol. {I}n 2{D}-{DIGE}/{MALDI}-{TOF} analysis, the {C}23{D} was found and identified only on phenol. {T}his set of results allowed us concluding that {S}. solfataricus 98/2 degrade phenol through the meta route.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {8},
  numero = {12},
  pages = {e82397},
  ISSN = {1932-6203},
  year = {2013},
  DOI = {10.1371/journal.pone.0082397},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061450},
}