@article{fdi:010061449, title = {{A}photic {N}-2 fixation in the {E}astern {T}ropical {S}outh {P}acific {O}cean}, author = {{B}onnet, {S}ophie and {D}ekaezemacker, {J}. and {T}urk-{K}ubo, {K}. {A}. and {M}outin, {T}. and {H}amersley, {R}.{M}. and {G}rosso, {O}. and {Z}ehr, {J}.{P}. and {C}apone, {D}.{G}.}, editor = {}, language = {{ENG}}, abstract = {{W}e examined rates of {N}-2 fixation from the surface to 2000 m depth in the {E}astern {T}ropical {S}outh {P}acific ({ETSP}) during {E}l {N}iño (2010) and {L}a {N}iña (2011). {R}eplicated vertical profiles performed under oxygen-free conditions show that {N}-2 fixation takes place both in euphotic and aphotic waters, with rates reaching 155 to 509 mu mol {N} m(-2) d(-1) in 2010 and 24 +/- 14 to 118 +/- 87 mu mol {N} m(-2) d(-1) in 2011. {I}n the aphotic layers, volumetric {N}-2 fixation rates were relatively low (< 1.00 nmol {N} {L}-1 d(-1)), but when integrated over the whole aphotic layer, they accounted for 87-90% of total rates (euphotic+ aphotic) for the two cruises. {P}hylogenetic studies performed in microcosms experiments confirm the presence of diazotrophs in the deep waters of the {O}xygen {M}inimum {Z}one ({OMZ}), which were comprised of non-cyanobacterial diazotrophs affiliated with nif{H} clusters 1{K} (predominantly comprised of alpha-proteobacteria), 1{G} (predominantly comprised of gamma-proteobacteria), and 3 (sulfate reducing genera of the delta-proteobacteria and {C}lostridium spp., {V}ibrio spp.). {O}rganic and inorganic nutrient addition bioassays revealed that amino acids significantly stimulated {N}-2 fixation in the core of the {OMZ} at all stations tested and as did simple carbohydrates at stations located nearest the coast of {P}eru/{C}hile. {T}he episodic supply of these substrates from upper layers are hypothesized to explain the observed variability of {N}-2 fixation in the {ETSP}.}, keywords = {{MILIEU} {MARIN} ; {EL} {NINO} ; {COUCHE} {EUPHOTIQUE} ; {FIXATION} {BIOLOGIQUE} {DE} {L}'{AZOTE} ; {OXYGENE} ; {MICROORGANISME} ; {ANALYSE} {QUANTITATIVE} ; {PROFIL} {VERTICAL} ; {PROFONDEUR} ; {LA} {NINA} ; {COUCHE} {APHOTIQUE} ; {OMZ}.{OXYGENE} {MINIMUM} {ZONE} ; {ZONE} {TROPICALE} ; {PACIFIQUE} {SUD}}, booktitle = {}, journal = {{P}los {O}ne}, volume = {8}, numero = {12}, pages = {e81265 [14 ]}, ISSN = {1932-6203}, year = {2013}, DOI = {10.1371/journal.pone.0081265}, URL = {https://www.documentation.ird.fr/hor/fdi:010061449}, }