@article{fdi:010061414,
  title = {{E}valuation of seafood toxicity in the {A}ustrales archipelago ({F}rench {P}olynesia) using the neuroblastoma cell-based assay},
  author = {{P}awlowiez, {R}. and {D}arius, {H}. {T}. and {C}ruchet, {P}. and {R}ossi, {F}. and {C}aillaud, {A}. and {L}aurent, {D}ominique and {C}hinain, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}iguatera fish poisoning ({CFP}), a disease caused by consuming fish that have accumulated ciguatoxins ({CTX}s) in their tissue, is regarded as the most prevalent form of intoxication in {F}rench {P}olynesia. {R}ecently, the {A}ustrales, one of the least affected archipelago until the early 1980s, has shown a dramatic increase in its incidence rates in 2009 with unusual {CFP} cases. {I}n the present work, potential health hazards associated with the proliferation of various marine phytoplankton species and the consumption of fish and marine invertebrates highly popular among local population were assessed in three {A}ustrales islands: {R}aivavae, {R}urutu and {R}apa. {E}xtracts from the marine dinoflagellates {G}ambierdiscus, {O}streospis and mat-forming cyanobacteria as well as fish, giant clams and sea urchin samples were examined for the presence of {CTX}s and palytoxin ({PLTX}) by using the neuroblastoma cell-based assay ({CBA}-{N}2a). {C}ytotoxic responses observed with both standards ({P}acific {CTX}-3{C} and {PLTX}) and targeted marine products indicate that {CBA}-{N}2a is a robust screening tool, with high sensitivity and good repeatability and reproducibility. {I}n {R}urutu and {R}aivavae islands, our main findings concern the presence of {CTX}-like compounds in giant clams and sea urchins, suggesting a second bio-accumulation route for {CFP} toxins in the ciguatera food chain. {I}n {R}apa, the potential {CFP} risk from {G}ambierdiscus bloom and fish was confirmed for the first time, with levels of {CTX}s found above the consumer advisory level of 0.01ng {P}acific {CTX}-1{B}g(-1) of flesh in three fish samples. {H}owever, despite the presence of trace level of {PLTX} in {O}streopsis natural assemblages of {R}apa, no sign of {PLTX} accumulation is yet observed in tested fish samples. {B}ecause this multi-toxinic context is likely to emerge in most {F}rench {P}olynesian islands, {CBA}-{N}2a shows great potential for future applications in the algal- and toxin-based field monitoring programmes currently on hand locally.},
  keywords = {ciguatera ; ciguatoxins ; palytoxin ; dinoflagellates ; cyanobacteria ; fish ; giant clam ; sea urchin ; neuroblastoma cell-based assay ; {F}rench {P}olynesia ; risk assessment ; {AUSTRALES} {ILES} ; {POLYNESIE} {FRANCAISE}},
  booktitle = {},
  journal = {{F}ood {A}dditives and {C}ontaminants. {P}art {A} : {C}hemistry {A}nalysis {C}ontrol {E}xposure and {R}isk {A}ssessment},
  volume = {30},
  numero = {3},
  pages = {567--586},
  ISSN = {1944-0049},
  year = {2013},
  DOI = {10.1080/19440049.2012.755644},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061414},
}