@article{fdi:010061392,
  title = {{A}nalysis of the black-chinned tilapia {S}arotherodon melanotheron heudelotii reproducing under a wide range of salinities : from {RNA}-seq to candidate genes},
  author = {{A}varre, {J}ean-{C}hristophe and {D}ugu{\'e}, {R}{\'e}mi and {A}lonso, {P}. and {D}iombokho, {A}. and {J}offrois, {C}. and {F}aivre, {N}. and {C}ochet, {C}hristophe and {D}urand, {J}ean-{D}ominique},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he black-chinned tilapia {S}arotherodon melanotheron heudelotii is an ecologically appealing model as it shows exceptional adaptive capacities, especially with regard to salinity. {I}n spite of this, this species is devoid of genomic resources, which impedes the understanding of such remarkable features. {D}e novo assembly of transcript sequences produced by next-generation sequencing technologies offers a rapid approach to obtain expressed gene sequences for non-model organisms. {I}t also facilitates the development of quantitative real-time {PCR} (q{PCR}) assays for analysing gene expression under different environmental conditions. {N}evertheless, obtaining accurate and reliable q{PCR} results from such data requires a number of validations prior to interpretation. {T}he transcriptome of {S}.melanotheron was sequenced to discover transcripts potentially involved in the plasticity of male reproduction in response to salinity variations. {A} set of 54 candidate and reference genes was selected through a digital gene expression ({DGE}) approach, and a de novo q{PCR} assay using these genes was validated for further detailed expression analyses. {A} user-friendly web interface was created for easy handling of the sequence data. {T}his sequence collection represents a major transcriptomic resource for {S}.melanotheron and will provide a useful tool for functional genomics and genetics studies.},
  keywords = {gene expression ; non-model organism ; reference gene ; salinity ; spermatogenesis ; tilapia ; {SENEGAL}},
  booktitle = {},
  journal = {{M}olecular {E}cology {R}esources},
  volume = {14},
  numero = {1},
  pages = {139--149},
  ISSN = {1755-098{X}},
  year = {2014},
  DOI = {10.1111/1755-0998.12148},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061392},
}