@article{fdi:010061369,
  title = {{P}utative panmixia in restricted populations of {T}rypanosoma cruzi isolated from wild {T}riatoma infestans in {B}olivia},
  author = {{B}arnab{\'e}, {C}hristian and {B}uitrago, {R}. and {B}r{\'e}mond, {P}hilippe and {A}liaga, {C}. and {S}alas, {R}. and {V}idaurre, {P}. and {H}errera, {C}. and {C}erqueira, {F}. and {B}osseno, {M}arie-{F}rance and {W}aleckx, {E}. and {B}reni{\`e}re, {S}imone {F}r{\'e}d{\'e}rique},
  editor = {},
  language = {{ENG}},
  abstract = {{T}rypanosoma cruzi, the causative agent of {C}hagas disease, is subdivided into six discrete typing units ({DTU}s; {T}c{I}-{T}c{VI}) of which {T}c{I} is ubiquitous and genetically highly variable. {W}hile clonality is the dominant mode of propagation, recombinant events play a significant evolutive role. {R}ecently, foci of wild {T}riatoma infestans have been described in {B}olivia, mainly infected by {T}c{I}. {H}ence, for the first time, we evaluated the level of genetic exchange within {T}c{I} natural potentially panmictic populations (single {DTU}, host, area and sampling time). {S}eventy-nine {T}c{I} stocks from wild {T}. infestans, belonging to six populations were characterized at eight microsatellite loci. {F}or each population, {H}ardy-{W}einberg equilibrium ({HWE}), linkage disequilibrium ({LD}), and presence of repeated multilocus genotypes ({MLG}) were analyzed by using a total of seven statistics, to test the null hypothesis of panmixia ({H}-0). {F}or three populations, none of the seven statistics allowed to rejecting {H}-0; for another one the low size did not allow us to conclude, and for the two others the tests have given contradictory results. {I}nterestingly, apparent panmixia was only observed in very restricted areas, and was not observed when grouping populations distant of only two kilometers or more. {N}evertheless it is worth stressing that for the statistic tests of "{HWE}", in order to minimize the type {I} error (i.e. incorrect rejection of a true {H}-0), we used the {B}onferroni correction ({BC}) known to considerably increase the type {II} error (i.e. failure to reject a false {H}-0). {F}or the other tests ({LD} and {MLG}), we did not use {BC} and the risk of type {II} error in these cases was acceptable. {T}hus, these results should be considered as a good indicator of the existence of panmixia in wild environment but this must be confirmed on larger samples to reduce the risk of type {II} error.},
  keywords = {{BOLIVIE}},
  booktitle = {},
  journal = {{P}lo{S} {O}ne},
  volume = {8},
  numero = {11},
  pages = {e82269},
  ISSN = {1932-6203},
  year = {2013},
  DOI = {10.1371/journal.pone.0082269},
  URL = {https://www.documentation.ird.fr/hor/fdi:010061369},
}