Cook S., Chung B. Y. W., Bass D., Moureau Grégory, Tang S. Y., McAlister E., Culverwell C. L., Glucksman E., Wang H., Brown T. D. K., Gould E. A., Harbach R. E., de Lamballerie Xavier, Firth A. E. (2013). Novel virus discovery and genome reconstruction from field RNA samples reveals highly divergent viruses in dipteran hosts. Plos One, 8 (11), p. e80720. ISSN 1932-6203.
Titre du document
Novel virus discovery and genome reconstruction from field RNA samples reveals highly divergent viruses in dipteran hosts
Année de publication
2013
Auteurs
Cook S., Chung B. Y. W., Bass D., Moureau Grégory, Tang S. Y., McAlister E., Culverwell C. L., Glucksman E., Wang H., Brown T. D. K., Gould E. A., Harbach R. E., de Lamballerie Xavier, Firth A. E.
Source
Plos One, 2013,
8 (11), p. e80720 ISSN 1932-6203
We investigated whether small RNA (sRNA) sequenced from field-collected mosquitoes and chironomids (Diptera) can be used as a proxy signature of viral prevalence within a range of species and viral groups, using sRNAs sequenced from wild-caught specimens, to inform total RNA deep sequencing of samples of particular interest. Using this strategy, we sequenced from adult Anopheles maculipennis s.l. mosquitoes the apparently nearly complete genome of one previously undescribed virus related to chronic bee paralysis virus, and, from a pool of Ochlerotatus caspius and Oc. detritus mosquitoes, a nearly complete entomobirnavirus genome. We also reconstructed long sequences (1503-6557 nt) related to at least nine other viruses. Crucially, several of the sequences detected were reconstructed from host organisms highly divergent from those in which related viruses have been previously isolated or discovered. It is clear that viral transmission and maintenance cycles in nature are likely to be significantly more complex and taxonomically diverse than previously expected.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
FRANCE ; CAMARGUE
Localisation
Fonds IRD [F B010061363]
Identifiant IRD
fdi:010061363
